Biochemical and Biophysical Research Communications
Apolipoprotein A-I induces IL-10 and PGE2 production in human monocytes and inhibits dendritic cell differentiation and maturation
Section snippets
Materials and methods
Reagents for cell culture, cytokines, and antibodies. All cultures were incubated in RPMI 1640 medium (Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY). The growth factors used in the primary cultures of DC precursors were recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF; provided by LG Biotech, Iksan, South Korea) and rhIL-4 (R&D Systems, Minneapolis, MN). Flow cytometric analysis for the determination of surface antigen
Apolipoprotein A-I impairs dendritic cell differentiation of monocytes
We attempted to determine whether or not the treatment of monocytes with apolipoprotein A-I (apoA-I) would affect their differentiation into dendritic cells (DC), in the presence of GM-CSF and IL-4. When antigen expressions were assessed by FACS analysis, CD1a expression on apoA-I-treated cells was appeared to have dramatically decreased in comparison with control DC, whereas CD14 expression in apoA-I-treated cells remained positive in a number of cells (Fig. 1A), indicating an arrest in the
Discussion
In this study, we demonstrated that apoA-I inhibits the differentiation from monocytes into DC in vitro, and also inhibits the ability of the mature DC to secrete IL-12 upon activation by CD40 and IFN-γ, while producing PGE2 and IL-10 during differentiation. Also, apoA-I inhibited immature DC functions, most notably, allogeneic T cell stimulation. ApoA-I-induced down-regulation of costimulatory molecules and MHC class II expression resulted in the inhibition of alloreactive T-cell activation
Acknowledgments
This work was supported by Grant, R11-2005-017-03001, of the Research Center for Women’s Diseases from the Korea Science and Engineering Foundation (KOSEF) and Grant, FG-3-1-04, of the 21C Frontier Functional Human Genome Project from the Ministry of Science and Technology (MOST), Korea.
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