Biochemical and Biophysical Research Communications
Metastin and its variant forms suppress migration of pancreatic cancer cells
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Materials and methods
Cell culture. Pancreatic cancer cell lines, AsPC-1, BxPC-3, Capan-2, CFPAC-1, PANC-1, and SUIT-2 were purchased from the American Type Culture Collection. Cells were cultured as monolayers in the appropriate medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37 °C in a humid atmosphere of 5% CO2/95% air. As for AsPC-1 and PANC-1, upon reaching 80% confluence, the medium was removed, the cells were washed in phosphate-buffered saline (PBS) and
Expression of KiSS1 and hOT7T175 in pancreatic cancer tissues
First, we measured the mRNA expression levels of KiSS1 and hOT7T175 in 30 pancreatic cancer tissues and in 5 adjacent normal pancreatic tissues. All the normal pancreatic tissues (5/5) and 14/30 of pancreatic ductal carcinoma tissues expressed KiSS1 mRNA (Fig. 1A). The expression level of KiSS1 mRNA in pancreatic cancer tissues was significantly lower than normal pancreatic tissues (p=0.018). In contrast, all the pancreatic cancer tissues (30/30) and normal pancreatic tissues (5/5) expressed
Discussion
In this study, we have demonstrated for the first time that hOT7T175 is expressed in pancreatic cancer tissues, but KiSS1 is less expressed when compared to normal pancreatic tissues. These results are in agreement with the analysis by other investigators in ovarian cancer, breast cancer, and colon cancer [2], [5]. Several reports indicated that KiSS1 and its receptor hOT7T175 are also highly expressed in placenta [4], [5]. The placenta is an invasive tissue, and there are similarities in the
Acknowledgements
This study was supported by a Grant-in-Aid for Scientific Research (#15390395) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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