American Journal of Orthodontics and Dentofacial Orthopedics
Online onlyBiomonitoring of mutagenicity and cytotoxicity in patients undergoing fixed orthodontic therapy
Section snippets
Subjects
The subjects of this study comprised a total of 23 healthy adults (10 men and 13 women) with a mean age of 18.5 ± 7 years who had submitted to orthodontic therapy at the Department of Orthodontics, São Paulo Methodist University, São Bernardo do Campo, Brazil. The fixed appliances consisted of an average of 4 to 8 bands and 20 bonded brackets. Brackets were provided by Abzil (São Paulo, Brazil): iron, 71%; nickel, 8%, and chromium, 19%. The arch wires used in this study were a nickel-titanium
Results
Figure 1 shows the frequencies of micronucleated cells in individuals undergoing orthodontic therapy. Before the beginning of orthodontic device exposure, the mean frequency of micronucleated cells was 0.04%. No statistically significant differences (P >0.05) were noticed either during or after orthodontic therapy. In the same way, an increase of other nuclear alterations were not observed throughout the experimental design, as depicted by the frequency of karyorrhexis, pyknosis, and
Discussion
The aim of this study was to employ the micronucleus test to assess chromosome damage and/or cellular death in adults who had submitted to orthodontic therapy. To the best of our knowledge, the approach has not been addressed in the literature so far.
The key advantage of the micronucleus assay is the relative ease of scoring, the limited costs and person-time required, and the precision obtained from scoring larger numbers of cells. The measurement of the frequency of micronuclei induced in
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Cited by (34)
Cytotoxicity, genotoxicity, and cellular metal accumulation caused by professionally applied fluoride products in patients with fixed orthodontic appliances: A randomized clinical trial.
2021, Journal of the World Federation of OrthodontistsBiomonitoring of children and adolescents using orthodontic appliances made of acrylic resins through micronucleus testing of exfoliated buccal and palatal mucosa cells
2021, American Journal of Orthodontics and Dentofacial OrthopedicsEvaluation of genotoxic damage in buccal mucosa cytome assays in Iraqi school children exposed to air pollutants emanating from oil fields
2021, Mutation Research - Genetic Toxicology and Environmental MutagenesisCitation Excerpt :Generally, an increased frequency of micronuclei (MN) being an efficient biomarker can be used for the diagnosis of genomic instability. Micronuclei are formed by eccentric chromosomes, chromatin fragments or whole chromosomes that fail to attach to the mitotic spindle and then are not included in the nucleus of the daughter cells, remaining as a round or oval cytoplasmic chromatin mass [33,34]. Furthermore, the MN cytome assay is a precise, inexpensive, noninvasive, and easy method for measuring the genotoxic and cytotoxic damage [35–37].
Corrosion potential in artificial saliva and possible genotoxic and cytotoxic damage in buccal epithelial cells of patients who underwent Ni-Cr based porcelain-fused-to-metal fixed dental prostheses
2018, Mutation Research - Genetic Toxicology and Environmental MutagenesisCitation Excerpt :Recent meta-analysis results have suggested that MN frequency in BEC can be used in prescreening and in the follow up of pre-cancerous oral lesions [45]. BMCyt assay is a preferred method among dentistry applications [46–52], however none of these studies cited here considered Ni-Cr PFM-FDPs. In the scientific literature, the study of Baricevic et al. [21] is the only in vivo human genotoxicity study concerning the use of Ni-Cr alloys used in fixed prosthodontic appliances.
The authors report no commercial, proprietary, or financial interest in the products or companies described in this article.
This study was supported by grants from São Paulo Research Foundation (Grant number: 07/00345-7). R.A.M. is a recipient of the São Paulo Research Foundation’s student fellowship and D.A.R. is a recipient of the CNPq fellowship.