Biophysical effects of high-energy pulsed ultrasound on human cells

doi:10.1016/S0301-5629(96)00151-2

Ultrasound in Medicine & Biology

Volume 22, Issue 9, 1996, Pages 1267-1275

https://doi.org/10.1016/S0301-5629(96)00151-2 Get rights and content

Abstract

Human benign and malignant cells of different human origin (pancreas, liver, kidney, pharynx, tongue, lip) were exposed to high-energy pulsed ultrasound (HEPUS) in vitro to evaluate the effects of various physical parameters and sonication conditions on cell viability. This included the number of pulses, focal pressure, pulse repetition rate, pulse shape, cell suspension volume, water level of the basin and cell density. Cell viability was found to depend significantly on the number of pulses (exponential), the focal pressure (linear) and the pulse repetition rate (minimum at 1 Hz). Other parameters showed no marked influence. Furthermore, electron microscopy revealed intracellular damage, and proliferation rates of cells surviving sonication were normal after HEPUS exposure. The experimental piezoelectric ultrasound transducer used in the experiments generated oscillating bipolar pulses with high negative pressure amplitudes. Measurements were made of the pulse shape and ultrasonic field of the experimental device and of a conventional lithotripter for comparison.

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Ultrasound in Medicine & Biology

Abstract

References (16)

Effect of shock waves on suspended and immobilized L1210 cells

Ultrasound Med Biol

Treatment of the dunning prostate rat tumor R3327-AT1 with pulsed high energy shock waves (PHEUS): Growth delay and histomorphologic changes

J Urol

Biological effects of shock waves: Cell disruption, viability, and proliferation of L1210 cells exposed to shock waves in vitro

Ultrasound Med Biol

Bioeffects of extracorporeal shock wave lithotripsy

Urol Clin North Am

High-energy shock waves suppress tumor growth in vitro and in vivo

J Urol

Cytotoxic effects of high-energy shock waves in different in vitro models: Influence of the experimental set-up

J Urol

Sensitivity of normal and malignant cells to shock waves

J. Stone Dis

Comparison of extracorporeal shockwave lithotripters

Cited by (12)

External Qi of Yan Xin Qigong differentially regulates the Akt and extracellular signal-regulated kinase pathways and is cytotoxic to cancer cells but not to normal cells

Ultrasound-induced cavitation damage to external epithelia of fish skin

Effects of non-thermal ultrasound on a fibroblast monolayer culture: Influence of pulse number and pulse repetition frequency

Sono-photodynamic combination therapy: A review on sensitizers

Sonodynamic therapy consisting of focused ultrasound and a photosensitizer causes a selective antitumor effect in a rat intracranial glioma model

Mechanism of photofrin-enhanced ultrasound-induced human glioma cell death

Original contributionBiophysical effects of high-energy pulsed ultrasound on human cells

Abstract

Ultrasound Med Biol

J Urol

Ultrasound Med Biol

Urol Clin North Am

J Urol

J Urol

Sensitivity of normal and malignant cells to shock waves

J. Stone Dis

Comparison of extracorporeal shockwave lithotripters

Original contribution
Biophysical effects of high-energy pulsed ultrasound on human cells