Immunohistochemical staining of Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues

doi:10.1016/S0046-8177(96)90006-4

Human Pathology

Volume 21, Issue 6, June 1990, Pages 607-612

https://doi.org/10.1016/S0046-8177(96)90006-4 Get rights and content

Abstract

We examined immunohistochemical staining with Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues. In cases of pancreatic cancer, its relation to histologic type was evaluated. Serous cystadenoma and atypical acinar cell nodules did not react with the Ha-ras oncogene product, and ductal cells and acinar cells in normal pancreas showed lower immunoreactivity than other benign or malignant lesions. However, the positive rate of islet cells in normal pancreas was almost the same in cases of pancreatic cancer. Strongest positivity was observed in cases of chronic pancreatitis, and islet cell tumors also showed high positive staining rates. In pancreatic cancer, the positive rate of well-differentiated adenocarcinomas was rather higher than that of poorly differentiated adenocarcinomas. Our study indicates that the Ha-ras oncogene p21 product does not correlate with neoplastic transformation in human pancreas, is not a useful marker for differentiating benign and malignant lesions, and cannot be used to determine the origin of differentiation in the pancreas.

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Original contributionImmunohistochemical staining of Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues

Abstract

Cell

Hum Pathol

Hum Pathol

Hum Pathol

Hum Pathol

Cell

Comparative biochemical properties of normal and activated human ras p21 protein

Nature

Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules

Monoclonal antibodies define differential ras gene expression in malignant and benign colonic diseases

Nature

Immunohistochemical detection of the ras oncogene p21 product in an experimental tumour and in human colorectal neoplasms

Br J Cancer

Immunocytochemical demonstration of p21 ras family oncogene product in normal mucosa and in premalignant and malignant tumours of the colorectum

Br J Cancer

ras oncogene p21 expression is increased in premalignant lesions and high grade bladder carcinoma

J Exp Med

Expression of ras oncogene p21 in prostate cancer

N Engl J Med

Immunohistochemical staining of colorectal tissues with monoclonal antibodies to ras oncogene p21 product and carbohydrate determinant antigen 19-9

J Clin Pathol

Antibodies to ras oncogene p21 proteins lack immunohistochemical specificity for neoplastic epithelium to human prostate tissue

Arch Pathol Lab Med

Immunohistochemical detection of ras oncogene p21 product in benign and malignant mammary tissue in man

J Clin Pathol

Histological demonstration of antigens reactive with anti-p21 ras monoclonal antibody (RAP-5) in human stomach cancers

JNCI

Original contribution
Immunohistochemical staining of Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues