Biochemical and Biophysical Research Communications
Nuclear localization of Galectin-3 in transformed thyroid cells: a role in transcriptional regulation
Section snippets
Experimental procedures
Oligodeoxynucleotide synthesis and DNA constructs. Oligodeoxynucleotides were purchased from MWG-Biotech, Ebersberg, Germany.
Plasmid encoding for recombinant TTF-1 homeodomain was described elsewhere [6]. Plasmid pTACAT3 contains the wild-type Tg promoter linked to the CAT gene and was already described [7]. Plasmid expressing protein TTF-1 was described elsewhere [8]. Plasmid encoding for Gal-3 was kindly provided by Dr. C. Hughes (National Institute for Medical Research, Mill Hill, London).
2D-PAGE analysis of nuclear extracts from Ki-mol and FRTL-5 cell lines
In order to investigate the presence of nuclear proteins differently expressed between the highly differentiated FRTL-5 rat thyroid cell line and the completely undifferentiated Ki-mol cell line, nuclear extracts were prepared as previously described [10]. The quality of the nuclear extracts was tested for the presence of the nuclear-specific transcription factor Pax-8 by Western blot analysis (data not shown). Then, expression profiles of nuclear proteins from FRTL-5 and Ki-mol cell lines were
Discussion
Thyroid cancers consist of diverse groups of neoplasms exhibiting different degrees of differentiation and aggressiveness [28]. In order to identify possible markers of dedifferentiation in thyroid cells, we used a differential proteomics approach comparing nuclear extracts from normal rat thyroid cell line FRTL-5 and transformed Ki-mol cell line.
Gal-3 was identified as expressed in the transformed cell line but not in the normal counterpart [19], [20], [21]. However, our results for the first
Acknowledgements
This work was supported by grants from the Italian National Research Council, Target Project on Biotechnology and from the Ministero per l’Università e la Ricerca Scientifica e Tecnologica (MIUR) to G.D. and from the University of Trieste to G.T.
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