Elsevier

Genomics

Volume 13, Issue 3, July 1992, Pages 565-574
Genomics

Genomic organization, chromosomal localization, and independent expression of human cyclin D genes

https://doi.org/10.1016/0888-7543(92)90126-DGet rights and content

Abstract

Murine cDNA clones for three cyclin D genes that are normally expressed during the G1 phase of the cell cycle were used to clone the cognate human genes. Bacteriophage and cosmid clones encompassing five independent genomic loci were partially sequenced and chromosomally assigned by an analysis of somatic cell hybrids containing different human chromosomes and by fluorescence in situ hybridization to metaphase spreads from normal peripheral blood lymphocytes. The human cyclin D1 gene (approved gene symbol, CCNDI) was assigned to chromosome band 11q13, cyclin D2 (CCNDI) to chromosome band 12p13, and cyclin D3 (CCND3) to chromosome band 6p21. Pseudogenes containing sequences related to cyclin D2 and cyclin D3 mapped to chromosome bands 11q13 and 6p21, respectively. Partial nucleotide sequence analysis of exons within each gene revealed that the authentic human cyclin D genes are more related to their mouse counterparts than to each other. These genes are ubiquitously transcribed in human tumor cell lines derived from different cell lineages, but are independently and, in many cases, redundantly expressed. The complex patterns of expression of individual cyclic D genes and their evolutionary conservation across species suggest that each family member may play a distinct role in cell cycle progression.

Reference (67)

  • GautierJ. et al.

    Cyclin is a component of maturation-promoting factor from Xenopus

    Cell

    (1990)
  • GautierJ. et al.

    Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+

    Cell

    (1988)
  • GiordanoA. et al.

    A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells

    Cell

    (1989)
  • HorsthemkeB. et al.

    Mechanism of i(6p) formation in retinoblastoma tumor cells

    Cancer Genet. Cytogenet.

    (1989)
  • KoffA. et al.

    Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family

    Cell

    (1991)
  • LabbeJ.C. et al.

    Purification of MPF from starfish: Identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation

    Cell

    (1989)
  • LewD.J. et al.

    Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast

    Cell

    (1991)
  • MatsushimeH. et al.

    Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle

    Cell

    (1991)
  • MorrisS.W. et al.

    Reassignment of the human CSF1 gene to chromosome 1p13–p21

    Blood

    (1991)
  • PinesJ. et al.

    Isolation of a human cyclin cDNA: Evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2

    Cell

    (1989)
  • RichardsonH.E. et al.

    An essential G1 function for cyclin-like proteins in yeast

    Cell

    (1989)
  • RosenthalE.T. et al.

    Selective translation of mRNA controls the pattern of protein synthesis during early development of the surf clam, Spisula solidissima

    Cell

    (1980)
  • StandartN. et al.

    Cyclin synthesis, modification and destruction during meiotic maturation of the starfish oocyte

    Dev. Biol.

    (1987)
  • SwensonK.I. et al.

    The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes

    Cell

    (1986)
  • ThomasP.S.

    Hybridization of denatured RNA transferred or dotted to nitrocellulose paper

  • XiongY. et al.

    Human D-Type cyclin

    Cell

    (1991)
  • AdelaideJ. et al.

    Chromosomal localization of the hst oncogene and its coamplification with the int-2 oncogene in a human melanoma

    Oncogene

    (1988)
  • ArnoldA. et al.

    Molecular cloning and chromosomal mapping of DNA rearranged with the parathyroid hormone gene in a parathyroid adenoma

    J. Clin. Invest.

    (1989)
  • AvivH. et al.

    Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

  • BuchkorichK. et al.

    The retinoblas toma protein is phosphorylated during specific phases of the cell cycle

    Cell

    (1989)
  • CollinsS.J.F. et al.

    Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds

  • CrossF.R.

    DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae

    Mol. Cell. Biol.

    (1988)
  • DouglassE.C. et al.

    A specific chromosomal abnormality in rhabdomyosarcoma

    Cytogenet. Cell Genet.

    (1987)
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    Sequence data from this article have been deposited with the Gen-Bank Data Library under Accession Nos. M88080, M88081, M88082, M88083, M88084, M88085, M88086, M88087, M86182, M86183.

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