Synergistic interaction of growth factors and albumin in regulating estradiol synthesis in breast cancer cells

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Abstract

Estradiol 17β-hydroxysteroid dehydrogenase acts to convert estrone to the biologically active estrogen, estradiol, in breast tumors and MCF-7 breast cancer cells in vitro. In this study we have examined the ability of albumin to influence the effect of growth factors (insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), transforming growth factor-α (TGFα) and cytokines (interleukin (IL)-1, IL-6) on estradiol 17β-hydroxysteroid dehydrogenase activity in MCF-7 cells. IGF-I (80 ng/ml) or albumin (30 μg/ml) stimulated estradiol 17β-hydroxysteroid dehydrogenase activity by 144% and 102% (p < 0.01). The combination of IGF-I and albumin, however, produced a marked (704%) synergistic stimulation of estradiol 17β-hydroxysteroid dehydrogenase activity. EGF or TGFα failed to stimulate estradiol 17β-hydroxysteroid dehydrogenase activity and no synergism with albumin was detected. IL-1 (10 ng/ml), but not IL-6, also stimulated estradiol 17β-hydroxysteroid dehydrogenase activity and acted synergistically with albumin to stimulate enzyme activity. MCF-7 cells were shown to specifically bind 125I-albumin and binding is increased by pretreatment of cells with IGF-I (80 ng/ml) for 48 h.

It is concluded that the synergism that results from treating MCF-7 cells with albumin and IGF-I may result from increased albumin uptake and subsequent biological effect.

References (37)

  • S. Corvera et al.

    J. Biol. Chem.

    (1989)
  • R.J. Davis et al.

    J. Biol. Chem.

    (1987)
  • D.M. Gersten et al.

    Med. Hypotheses

    (1989)
  • H.T. Haigler et al.

    J. Biol. Chem.

    (1980)
  • V.H.T. James et al.

    Steroids

    (1987)
  • R. Koistinen et al.

    Biochem. Biophys. Res. Commun.

    (1990)
  • J.A. Leal et al.

    Steroids

    (1991)
  • R. Melsert et al.

    Mol. Cell. Endocrinol.

    (1989)
  • A.M. Nakhla et al.

    Biochem. Biophys. Res. Commun.

    (1988)
  • T. Peters

    Adv. Protein Chem.

    (1985)
  • M.R. Shalaby et al.

    Cell. Immunol.

    (1989)
  • A. Singh et al.

    Cancer Lett.

    (1989)
  • A. Singh et al.

    Mol. Cell. Endocrinol.

    (1992)
  • A. Vermeulen et al.

    Eur. J. Cancer Clin. Oncol.

    (1986)
  • M.J. Villacampa et al.

    Biochem. Biophys. Res. Commun.

    (1984)
  • E.F. Adams et al.

    Int. J. Cancer

    (1991)
  • J.C. Bonner et al.

    J. Cell. Physiol.

    (1990)
  • R.C. Bonney et al.

    Clin. Endocrinol.

    (1983)
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