Cell
Volume 72, Issue 5, 12 March 1993, Pages 729-739
Journal home page for Cell

Article
The oncoprotein Bcl-3 directly transactivates through κB motifs via association with DNA-binding p50B homodimers

https://doi.org/10.1016/0092-8674(93)90401-BGet rights and content

Abstract

Bcl-3 is an IκB-related protein with ankyrin repeat motifs. Its gene is located at a site of recurrent translocations in a subset of B cell chronic lymphocytic leukemias. Bcl-3 associates tightly with p50B (NFKB2, p52) homodimers in cells, and together these proteins form a ternary complex with DNA at κB sites. Such an association functionally leads to a novel and potent form of transactivation through the κB motif: the tethering of Bcl-3 to DNA via the p50B homodimers allows Bcl-3 to transactivate directly, while p50B homodimers alone cannot. Transactivation mediated by Bcl-3 requires two cooperating domains located amino- and carboxyterminal to the ankyrin domain. Bcl-3 is localized to the nucleus, and a Bcl-3-p50B complex is detected in certain lymphoid cells. Our data reveal a novel role for Bcl-3, distinct from that of the inhibitor IκB. The results have implications for tumorigenesis.

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    Present address: Laboratoire d'Etude des Métastases et de l'Invasion Tumorale, Pathologie B23, Domaine du Sart-Tilman, Université de Liège, 4000 Liège, Belgium.

    Permanent address: Servizio di Microbiologia del Complesso Convenzionato Università—U.L.S.S. 21, Via Ospedale 2, 35100 Padua, Italy.

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