Cell
Volume 42, Issue 2, September 1985, Pages 581-588
Journal home page for Cell

Article
Activation of a novel human transforming gene, ret, by DNA rearrangement

https://doi.org/10.1016/0092-8674(85)90115-1Get rights and content

Abstract

A novel transforming gene was detected by transfection of NIH 3T3 cells with human lymphoma DNA. The tumor DNA induced a single focus in primary transfections, whereas DNAs of transformed NIH cells induced transformation with high efficiencies in secondary and tertiary assays. Molecular clones spanning about 37 kb of human sequence were isolated from tertiary transformant DNA. Blot hybridization indicated that the transforming gene consisted of two segments that were unlinked in both normal human and primary lymphoma DNAs. The two segments of human DNA were cotranscribed in transformed NIH cells but not in any human cells examined. The transforming gene thus appeared to be activated by recombination between two unlinked human DNA segments, possibly by cointegration during transfection.

References (44)

  • C.S. Cooper et al.

    Characterization of human transforming genes from chemically transformed, teratocarcinoma and pancreatic carcinoma cell lines

    Cancer Res.

    (1984)
  • C.S. Cooper et al.

    Molecular cloning of a new transforming gene from a chemically transformed human cell line

    Nature

    (1984)
  • G.M. Cooper

    Cellular transforming genes

    Science

    (1982)
  • G.M. Cooper et al.

    Transforming activity of DNA of chemically transformed and normal cells

    Nature

    (1980)
  • D. Defeo et al.

    Analysis of two divergent rat genomic clones homologous to the transforming gene of Harvey murine sarcoma virus

  • C.J. Der et al.

    Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses

  • A. Diamond et al.

    Identification and molecular cloning of the human Blym transforming gene activated in Burkitt's lymphomas

    Nature

    (1983)
  • O. Fasano et al.

    New human transforming genes detected by a tumorigenicity assay

    Mol. Cell. Biol.

    (1984)
  • M. Goldfarb et al.

    Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma

    Nature

    (1982)
  • A. Hall et al.

    Identification of transforming gene in two human sarcoma cell lines as a new member of the ras gene family located on chromosome 1

    Nature

    (1983)
  • T. Hercend et al.

    Identification of a clonally restricted 90 kD heterodimer on two human cloned natural killer cell lines

    J. Exp. Med.

    (1983)
  • B. Hohn et al.

    Packaging recombinant DNA molecules into bacteriophage particles in vitro

  • Cited by (0)

    View full text