Correlation of metabolic stability and altered quaternary structure of oncoprotein p53 with cell transformation☆
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Prognostic and predictive value of tumoral DNA damage repair protein expression in patients with resected pancreatic cancer
2023, Clinics and Research in Hepatology and GastroenterologyReassessment of p53 immunohistochemistry thresholds in invasive high grade bladder cancer shows a better correlation with TP53 and FGFR3 mutations
2020, Pathology Research and PracticeCitation Excerpt :In the past, the immunohistochemical assessment of p53 protein expression in HGUC tumour cells focused on the total percentage of tumour nuclei with immunoexpression, without consideration of the complete lack of nuclear immunoexpression; a designation as “p53 positive” was applied when a proportion of tumour nuclei (between 10–50 %) expressed p53. Of course, this is not entirely accurate from a biological perspective, as it is known that missense mutations typically lead to a stability and accumulation of the p53 protein within the majority of tumour nuclei [45,46] and less commonly, complete lack of expression as a result of nonsense mutations. In light of this, members of our group recently described an alternative contemporary p53 immunohistochemistry scoring method in HGUC (abnormal vs. wild type) that has been shown to correlate with oncologic outcome [31] and that is more biologically congruent however, correlation with TP53 mutation status was not previously explored.
Pitfalls in molecular diagnostics
2019, Seminars in Diagnostic PathologyCitation Excerpt :Wild-type p53 is relatively unstable in the absence of cellular stress and has a short half-life, and is thus expected to have weak or scattered reactivity to antibody staining.41 In contrast, missense mutations in TP53 often create a protein with a longer half-life, resulting in strong and diffuse staining.42,43 However, a complete lack of staining may also be observed, which is thought to correspond to nonsense mutations resulting in a truncated, non-immunoreactive protein.44
p53 alteration independently predicts poor outcomes in patients with endometrial cancer: A clinicopathologic study of 131 cases and literature review
2010, Gynecologic OncologyCitation Excerpt :Single base missense mutations in the highly conserved regions are the most common lesion in the gene. This makes p53 protein more stable than wild-type protein, leading to prolonged half-life and subsequently resulting in accumulation [8,9]. These mutations are associated with increased expression of p53 protein in the nucleus as shown by Immunohistochemical analysis [10–12].
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This study was supported by Grants SFB322 A1 and Mo309/2-1 from the Deutsche Forschungsgemeinschaft to M.M.