A general colorimetric procedure for the estimation of enzymes which are linked to the NADH/NAD+ system
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2021, Biomedicine and PharmacotherapyCitation Excerpt :The supernatant thus achieved was employed in various enzyme assays [22,23]. Kits from Vitro Scient (Cairo, Egypt) were employed in keeping with the protocol proposed by Whitaker [24] for the measurement of serum lactate dehydrogenase (LDH) via a kinetic approach, while kits (Vitro Scient) applied in keeping with the protocol by Tousson and colleagues [7] were used to measure serum creatine kinase (CK) via an akinetic approach. Furthermore, an assay kit from Bioassay Systems (Hayward, CA, USA) was used according to the protocol recommended by Bishop and colleagues [25] to measure CK-muscle brain (CK-MB) activity in serum.
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2014, Toxicology ReportsCitation Excerpt :The increase in absorbance is measured spectrophotometrically at 340 nm at 1 min intervals for 3 min. Serum total LDH activity was calculated as (U/L) according to the method of Whitaker [21]. TNF-α in the cardiac homogenate was assayed using enzyme-linked immunosorbent assay (ELISA) using a microplate reader (Spectra III Classic, Tecan, Salzburg, Austria) as previously described [22].
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2014, Chemico-Biological InteractionsCitation Excerpt :An experienced pathologist blinded to the study groups examined the sections obtained from cardiac tissues. The lactate dehydrogenase activity in the rat serum was estimated using a commercially available LDH kit (Sigma–Aldrich, Bayouni Trading Co. Ltd., Al-Khobar, Saudi Arabia), as described previously [73]. In this assay, lactate dehydrogenase (LDH) catalyzes the reduction of pyruvate to lactate in the presence of reduced nicotinamide adenine dinucleotide (NADH) at pH 7.5.
Does clozapine induce myocarditis, myocardial oxidative stress and DNA damage in rats?
2014, Egyptian Journal of Forensic SciencesCitation Excerpt :The rate of NADH formation, measured at 340 nm, is, therefore, directly proportional to creatine kinase activity. Lactate dehydrogenase activity was estimated in serum by commercially available LDH kit (Linear Chemicals, S.L., UK) according to the method of Whitaker.21 Using this method, lactate dehydrogenase (LDH) catalyzes the reduction of pyruvate to lactate in the presence of reduced nicotinamide adenine dinucleotide (NADH) at pH 7.5.