Alkaline phosphatase in HT-29, a human colon cancer cell line: Influence of sodium butyrate and hyperosmolality

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Abstract

HT-29, a cell line derived from a human colon carcinoma, exhibits very low alkaline phosphatase activity. The enzyme is thermolabile and is of the intestinal type. Hyperosmolality and/or sodium butyrate induce increased levels of activity. The increase is most pronounced with HT-29 cells growing in hyperosmolar medium containing sodium butyrate. Under these conditions specific activity rises over 1000-fold. The effect of hyperosmolality is blocked by cycloheximide and that of sodium butyrate by thymidine, cordycepin, and cycloheximide. By contrast to other human cancer cell lines, the enzyme of HT-29 is not influenced by cell density and glucocorticoid hormones. 5-Bromo-2′-deoxyuridine and inhibitors of DNA synthesis cause a slight increase in specific activity.

References (64)

  • F. Herz et al.

    Arch. Biochem. Biophys

    (1962)
  • C.-W. Lin et al.

    J. Biol. Chem

    (1972)
  • G.J. Doellgast et al.

    Clin. Chim. Acta

    (1977)
  • W.H. Fishman

    Amer. J. Med

    (1974)
  • M.E. Forgue-Lafitte et al.

    Mol. Cell. Endocrinol

    (1979)
  • M.J. Griffin et al.

    Arch. Biochem. Biophys

    (1974)
  • O.H. Lowry et al.

    J. Biol. Chem

    (1951)
  • H.M. Nitowsky et al.

    Biochem. Biophys. Res. Commun

    (1963)
  • F. Herz

    Arch. Biochem. Biophys

    (1973)
  • H.M. Nitowsky et al.

    Biochem. Biophys. Res. Commun

    (1963)
  • F. Herz et al.

    Biochim. Biophys. Acta

    (1973)
  • F. Herz et al.

    Arch. Biochem. Biophys

    (1979)
  • S.I. Deutsch et al.

    Biochim. Biophys. Acta

    (1977)
  • B.Z. Littlefield et al.

    Arch. Biochem. Biophys

    (1980)
  • M.C. Robertson et al.

    Biochim. Biophys. Acta

    (1977)
  • F. Koch et al.

    Arch. Biochem. Biophys

    (1977)
  • D.N. Wheatley

    Exp. Cell Res

    (1974)
  • R. Prizont et al.

    Gastroenterology

    (1975)
  • M.J. McKenna et al.

    Biochem. J

    (1979)
  • D.J. Goldstein et al.
  • L.E. Seargent et al.

    Nature (London)

    (1979)
  • H. Van Belle et al.

    Clin. Chem

    (1977)
  • W.H. Fishman et al.

    Enzymologia

    (1971)
  • R.A. Mulivor et al.
  • W.W. Rosen et al.

    Arch. Int. Med

    (1975)
  • W.H. Fishman et al.
  • S.L. Ehrmeyer et al.

    Cancer Res

    (1978)
  • T. Hada et al.
  • F.J. Benham et al.
  • S.-I. Tokumitsu et al.

    Cancer Res

    (1979)
  • R.H. Kottel et al.

    Scand. J. Immunol

    (1978)
  • J. Fogh et al.
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