Modification of oligosaccharide- lipid synthesis and protein glycosylation in glucose-deprived cells

doi:10.1016/0003-9861(80)90115-0

Archives of Biochemistry and Biophysics

Volume 205, Issue 2, December 1980, Pages 330-339

https://doi.org/10.1016/0003-9861(80)90115-0 Get rights and content

Abstract

Incubation of vesicular stomatitis virus-infected glucose-starved baby hamster kidney cells with [³⁵S]methionine results in the synthesis of all viral proteins. However, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tryptic peptide mapping, the G protein is abnormally glycosylated. Metabolic labeling of the oligosaccharide-lipid precursors with [³H]mannose for 15 min, followed by Chromatographic and enzymatic analysis, indicates that the radiolabeled lipid-linked oligosaccharides are devoid of glucose in contrast to the glucosylated oligosaccharide-lipids synthesized by cells grown in the presence of glucose. Also, in contrast to control cells, examination of the glycopeptide fraction reveals the presence of [³H]mannose-labeled glycopeptides which are resistant to erado-β-N-acetylglucos-aminidase H and are smaller in size than glycopeptides from mature vesicular stomatitis virus. In order to observe these effects, a minimum time of 5 h of glucose deprivation is necessary and the addition of 55 μm glucose or mannose to the medium reverses these effects. These results indicate that vesicular stomatitis virus-infected BHK cells deprived of glucose are unable to glucosylate the oligosaccharide-lipid intermediates and, consequently, are unable to glycosylate the G protein normally.

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Citation Excerpt :
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Modification of oligosaccharide- lipid synthesis and protein glycosylation in glucose-deprived cells

Abstract

Biochim. Biophys. Acta

J. Biol. Chem

J. Biol. Chem

Arch. Biochem. Biophys

Cell

Virology

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

Cell

J. Biol. Chem

Anal. Biochem

J. Biol. Chem