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Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09

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Abstract

Fermentation conditions for the production of bacterial cellulose (BC) by a newly isolated Gluconacetobacter hansenii UAC09 were optimized. The effect of various carbon and nitrogen sources on production of BC was determined. The addition of ethanol (1.5%) and acetic acid (1.0%) enhanced BC production by more than three times compared to standard Hestrin and Schramm medium. The strain was able to produce maximum BC (7.40 g l−1) with 4% glucose, 8% corn steep liquor (CSL) at 27 ± 1°C and a pH of 5.5 under stationary conditions. The BC films produced under various conditions were tested for their structural similarity using Fourier transform-infra red analysis and were found to be similar to each other irrespective of medium composition. Scanning electron microscopy images of BC revealed a porous structure with extensively interwoven microfibrils.

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Acknowledgments

The first author wishes to acknowledge CSIR, New Delhi for awarding a Senior Research Fellowship. The authors are thankful to the Director, CFTRI, Mysore, and to the Head of the Food Microbiology Department, for providing facilities and for their support and help to carry out this study.

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Authors and Affiliations

  1. Food Microbiology Department, Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India

    Mahadevaswamy Usha Rani & Anu Appaiah

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  1. Mahadevaswamy Usha Rani
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  2. Anu Appaiah
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Correspondence to Anu Appaiah.

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Rani, M.U., Appaiah, A. Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09. Ann Microbiol 61, 781–787 (2011). https://doi.org/10.1007/s13213-011-0196-7

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  • DOI: https://doi.org/10.1007/s13213-011-0196-7

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