Abstract
Prostate cancer (PCa) is the most common non-cutaneous cancer in the United States. There is currently a lack of safe and effective radiosensitizers that can enhance the effectiveness of radiation treatment (RT) for Pca. Clonogenic assay, PCNA staining, Quick Cell Proliferation assay, TUNEL staining and caspase-3 activity assay were used to assess proliferation and apoptosis in DU145 Pca cells. RT-PCR/IHC were used to investigate the mechanisms. We found that the percentage of colonies, PCNA staining intensity, and the optical density value of DU145 cells were decreased (RT/GT vs. RT). TUNEL + cells and the relative caspase-3 activity were increased (RT/GT vs. RT). Compared to RT, the anti-proliferative effect of RT/GT correlated with increased expression of the anti-proliferative molecule p16. Compared to RT, the pro-apoptotic effect of RT/GT correlated with decreased expression of the anti-apoptotic molecule Bcl-2. GT enhances RT sensitivity of DU145 by inhibiting proliferation and promoting apoptosis.
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Acknowledgments
This study was supported by grants from Des Moines University for Yujiang Fang, MD. PhD. (IOER 05-14-01 and IOER 112-3749). Andrew C. Schroeder, B. S. was supported by Mentored Research Program from Des Moines University (IOER 112-3113).
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Schroeder, A.C., Xiao, H., Zhu, Z. et al. A Potential Role for Green Tea as a Radiation Sensitizer for Prostate Cancer. Pathol. Oncol. Res. 25, 263–268 (2019). https://doi.org/10.1007/s12253-017-0358-4
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DOI: https://doi.org/10.1007/s12253-017-0358-4