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Circulating microparticles in patients with coronary heart disease and its correlation with interleukin-6 and C-reactive protein

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Abstract

Microparticles (MPs) are vesicles released from activated or apoptotic cells. MP derive from various cells, most notably platelets, but also leucocytes, lymphocytes, erythrocytes, and endothelial cells. The aim of this study was to investigate endothelial MP (EMP), platelet MP (PMP), lymphocyte MP and monocyte MP and TF-positive MPs (TF+ MPs) in patients with coronary heart disease (CHD), and to evaluate the correlation of these MPs with Interleukin-6 (IL-6) and C-reactive protein (CRP). Different cell-derived MPs and TF+ MPs were analyzed by flow cytometry in 40 patients with myocardial infarction (MI), 30 unstable angina (UA), 20 stable angina (SA) and 20 healthy individuals, and IL-6 and CRP were determined by ELISA and special protein analyzer, respectively. Compared with SA and control, EMP and PMP was significantly elevated in MI and UA (P < 0.001), and TF+ MPs was significantly elevated in MI and UA (P < 0.001). EMP and PMP correlated with IL-6 (r = 0.822, P < 0.001 and r = 0.567, P < 0.001; respectively) or CRP level (r = 0.597, P < 0.001 and r = 0.66, P < 0.001; respectively). Different cell-derived MPs in CHD may indicate the different pathophysiological changes in vessels, and MPs may both participate in the development of thrombosis and enhance the vascular inflammation.

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Acknowledgments

The authors thank the anesthesiology department of staff for their invaluable help, support, and patience. We also thank all our patients that, with their consent, made this study possible. Supported by Heilongjiang Province post-doctor Fund (LBH-Q11069).

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Authors and Affiliations

  1. Department of Laboratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, People’s Republic of China

    Ying Cui, Lihui Zheng, Ming Jiang, Ru Jia, Xiao Zhang, Qishan Quan, Guiqin Du, Dongjin Shen, Xiaodan Zhao, Wenying Sun, Hongwei Xu & Lijuan Huang

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  1. Ying Cui
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  2. Lihui Zheng
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  3. Ming Jiang
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  4. Ru Jia
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  5. Xiao Zhang
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  7. Guiqin Du
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  10. Wenying Sun
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  11. Hongwei Xu
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  12. Lijuan Huang
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Correspondence to Lijuan Huang.

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Cui, Y., Zheng, L., Jiang, M. et al. Circulating microparticles in patients with coronary heart disease and its correlation with interleukin-6 and C-reactive protein. Mol Biol Rep 40, 6437–6442 (2013). https://doi.org/10.1007/s11033-013-2758-1

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  • DOI: https://doi.org/10.1007/s11033-013-2758-1

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