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DNA repair enzyme polymorphisms and oxidative stress in a Turkish population with gastric carcinoma

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Abstract

Although the developmental stages of gastric carcinoma are still not clear, the constantly generated reactive oxygen and nitrogen species (ROS/RNS) may contribute to the process of carcinogenesis by interacting with DNA. 8-oxoguanine DNA glycosylase-1 (OGG1) is an enzyme involved in base excision repair of 8-oxoguanine that is one of the premutagenic lesions generated by ROS in DNA. The bulky adducts, are recognized and repaired by nucleotid excision repair (NER) enzymes, including xeroderma pigmentosum C and D (XPC, XPD). Eligible 106 gastric cancer patients and 116 cancer-free individuals constituted the study and control groups, respectively. Association between OGG1 Ser326Cys, XPC Lys939Gln, XPD Lys751Gln polymorphisms and the susceptibility tho cancer and the oxidative stress status were evaluated. DNA was extracted from peripheral blood cells and genotypes were determined by using PCR–RFLP. Serum nitric oxide, albumin concentrations, total antioxidant status and Helicobacter pylori IgG were determined. Serum albumin and nitric oxide of cancer patients were lower than that of the controls (P < 0.05). None of the evaluated polymorphisms or Helicobacter pylori IgG seropositivity associated with increased risk of gastric cancer, despite of the increased oxidative stress in cancer patients.

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Acknowledgments

This study was supported by Gazi University, Scientific Research Projects Division, 02/2007-26 and The Scientific and Technological Research Council of Turkey, 107S363.

Conflict of interest

All authors disclose that they have no any financial or personal relationships with other people or organizations. There is no direct financial interest in the subject matter or materials discussed in the manuscript that could inappropriately influence the work submitted. Investigators also disclose any potential conflicts to participants in clinical trials and other studies and state in the manuscript whether they have done so.

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Authors and Affiliations

  1. Department of Toxicology, Faculty of Pharmacy, Gazi University, 06330, Hipodrom, Ankara, Turkey

    Ayse Basak Engin, Bensu Karahalil & Ali Esat Karakaya

  2. Department of General Surgery, Faculty of Medicine, Gazi University, 06500, Beşevler, Ankara, Turkey

    Atilla Engin

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  1. Ayse Basak Engin
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  2. Bensu Karahalil
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  3. Atilla Engin
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  4. Ali Esat Karakaya
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Correspondence to Ayse Basak Engin.

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Engin, A.B., Karahalil, B., Engin, A. et al. DNA repair enzyme polymorphisms and oxidative stress in a Turkish population with gastric carcinoma. Mol Biol Rep 38, 5379–5386 (2011). https://doi.org/10.1007/s11033-011-0690-9

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  • DOI: https://doi.org/10.1007/s11033-011-0690-9

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