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Association study of single nucleotide polymorphisms in pre-miRNA and rheumatoid arthritis in a Han Chinese population

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Abstract

The aim of this study was to perform an association study between two single nucleotide polymorphisms (SNPs) rs2910164 G>C and rs3746444 T>C in pre-miRNA (hsa-mir-146a and hsa-mir-499) and rheumatoid arthritis (RA) in the Han Chinese population. 208 Han Chinese patients with RA and 240 healthy controls were recruited in this study. The SNPs was genotyped by polymerase chain reaction-restriction fragment length polymorphism. Anti-cyclic citrullinated peptide (anti-CCP) antibody was measured by enzyme linked immunosorbent assay and rheumatoid factor (RF) was measured by rate nephelometry. The genotype frequencies between cases and controls were compared by χ2 analysis. No significant association between the SNPs (rs2910164 and rs3746444) and RA was observed (P = 0.631 and 0.775, respectively), and the SNPs did not show any association with the RF-positive (P = 0.631 and 0.775, respectively). However, there was a significant difference on the level of anti-CCP antibody between different genotypes in rs3746444 (P = 0.007). The heterozygote CT had significantly higher level of anti-CCP antibody compared with homozygote CC and TT (P = 0.054 and 0.003, respectively). We first investigated the association between the SNPs (rs2910164 G>C and rs3746444 T>C) in the pre-miRNA (hsa-mir-146a and hsa-mir-499) and RA in a Han Chinese population. We did not find a significant association between the SNPs and the susceptibility to RA, while the SNP rs3746444 may affect anti-CCP antibody production.

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Acknowledgments

The authors thank the participating RA patients and their families. The authors acknowledge the grant support from the National Natural Science Foundation of China (Grant No. 30670819, 30900658, 30772051 and 30950010).

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Authors and Affiliations

  1. Division of Clinical Immunology, Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Bin Yang, Jun Long Zhang, Dong Dong Li, Jie Chen, Zhuo Chun Huang, Bei Cai, Xing Bo Song, Li Xin Li, Bin Wu Ying & Lan Lan Wang

  2. Department of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Yun Ying Shi

  3. Division of Molecular Biology, Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Bin Wu Ying

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  1. Bin Yang
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Correspondence to Bin Wu Ying or Lan Lan Wang.

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LanLan Wang and BinWu Ying contributed equally to this work.

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11033_2010_633_MOESM1_ESM.doc

Supplementary Figure 1f: The relation between anti-CCP antibody and Frequency distribution of SNP rs2910164 G > C genotypes in RA patients (A) and female RA patients (B) (A) The median of anti-CCP antibody in genotype CC was 119.16 RU/ml (9.51–196.65); The median of anti-CCP antibody in genotype CG was 135.53 RU/ml (17.55–235.75); The median of anti-CCP antibody in genotype GG was 156.06 RU/ml (18.35–273.51); There was no significant difference between different genotypes (P = 0.554). (B) The median of anti-CCP antibody in genotype CC was 106.49 RU/ml (9.69–222.87). The median of anti-CCP antibody in genotype CG was 120.47 RU/ml (16.33–235.66). The median of anti-CCP antibody in genotype GG was 151.35 RU/ml (11.09–287.61). There was no significant difference between different genotypes (P = 0.736). (DOC 115 kb)

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Yang, B., Zhang, J.L., Shi, Y.Y. et al. Association study of single nucleotide polymorphisms in pre-miRNA and rheumatoid arthritis in a Han Chinese population. Mol Biol Rep 38, 4913–4919 (2011). https://doi.org/10.1007/s11033-010-0633-x

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