Abstract
Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxytyrosol. In this study, oleuropein and hydroxytyrosol, major phenolic compound of olive oil, was studied for its effects on growth in MCF-7 human breast cancer cells using assays for proliferation (MTT assay), cell viability (Guava ViaCount assay), cell apoptosis, cellcycle (flow cytometry). Oleuropein or hydroxytyrosol decreased cell viability, inhibited cell proliferation, and induced cell apoptosis in MCF-7 cells. Result of MTT assay showed that 200 μg/mL of oleuropein or 50 μg/mL of hydroxytyrosol remarkably reduced cell viability of MCF-7 cells. Oleuropein or hydroxytyrosol decrease of the number of MCF-7 cells by inhibiting the rate of cell proliferation and inducing cell apoptosis. Also hydroxytyrosol and oleuropein exhibited statistically significant block of G1 to S phase transition manifested by the increase of cell number in G0/G1 phase.
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References
Abaza L, Taamalli W, Zarrouk M et al (2005) Natural antioxidant composition as correlated to stability of some Tunisian virgin olive oils. Rivista Italiano Delle Sostanze Gresse 132:12–18
Babich H, Visioli F (2003) In vitro cytotoxicity to human cells in culture of some phenolics from olive oil. Farmaco 58:403–407
Caponio F, Alloggio V, Gomes T (1999) Phenolic compounds of virgin olive oil: influence of paste preparation techniques. Food Chem 64:203–209
Corona G, Deiana M, Incani A et al (2007) Inhibition of p38/CREB phosphorylation and COX-2 expression by olive oil polyphenols underlies their anti-proliferative effects. Biochem Biophys Res Commun 362:606–611
Elisa T, Marco G, Garden T et al (2005) The phenolic compounds of olive oil: structure, biological activity and beneficial effects on human health. Nutr Res Rev 18:98–112
Fabiani R, De Bartolomeo A, Rosignoli P et al (2002) Cancer chemoprevention by hydroxytyrosol isolated from virgin olive oil through G1 cell cycle arrest and apoptosis. Eur J Cancer Prev 11:351–358
Fabiani R, Bartolomeo AD, Rosignoli P et al (2006) Virgin olive oil phenols inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis and differentiation. J Nutr 136:614–619
Georg F, Surajit D, Volker F et al (2007) Blockade of hedgehog signaling inhibits pancreatic cancer invasion and metastases: a new paradigm for combination therapy in solid cancers. Cancer Res 67:2187–2196
Hamdi K, Hamdi RC (2005) Oleuropein, a non-toxic olive iridoid, is an anti-tumor agent and cytoskeleton disruptor. Biochem Biophys Res Commun 334:769–778
Keceli T, Gordon HM (2001) The antioxidant activity and stability of the phenolic fraction of green olives and extra virgin olive oil. J Sci Food Agric 81:1391–1396
Keys A, Aravanis C, Van Buchem FSP et al (1981) The diet and all-causes death rate in the seven countries study. Lancet 8237:58–61
La Vecchia C, Negri E, Franceschi S et al (1998) Olive oil, other dietary fats, and the risk of breast cancer (Italy). Cancer Causes Control 6:545–550
Le Tutour B, Guedon D (1992) Antioxidative activities of Olea europaea leaves and related phenolic compounds. Phytochemistry 31:1173–1178
Litridou M, Linssen J, Schols H et al (1997) Phenolic compounds in virgin olive oils: fractionation by solid phase extraction and antioxidant activity assessment. J Sci Food Agric 74:169–174
Martin-Moreno JM, Willett WC, Gorgojo L et al (1994) Dietary fat, olive oil intake and breast cancer risk. Int J Cancer 58:774–780
O’Dowd Y, Driss F, Dang PM et al (2004) Antioxidant effect of hydroxytyrosol, a polyphenol from olive oil: scavenging of hydrogen peroxide but not superoxide anion produced by human neutrophils. Biochem Pharmacol 68:2003–2008
Owen RW, Mier W, Giacosa A et al (2000a) Phenolic compounds and squalene in olive oils: the concentration and antioxidant potential of total phenols, simple phenols, secoiridoids, lignans and squalene. Food Chem Toxicol 38:647–659
Owen RW, Giacosa A, Hull WE et al (2000b) The antioxidant anticancer potential of phenolic compounds isolated from olive oil. Eur J Cancer 36:1235–1247
Paula PC, Pereira AM, Silvério C et al (2007) Fiber, beans, olive oil and breast cancer-toxic or beneficial. Toxicol Lett 172:S199
Ryan D, Antolovich M, Prenzler P et al (2002) Biotransformations of phenolic compounds in Olea europaea L. Sci Hortic 92:147–176
Servili M, Baldioli M, Selvaggini R et al (1999) Phenolic compounds of olive fruit: one- and two-dimensional nuclear magnetic resonance characterization of nuzhenide and distribution in the constitutive parts of fruit. J Agric Food Chem 47:12–18
Tuck LK, Hayball JP (2002) Major phenolic compounds in olive oil: metabolism and health effects. J Nutr Biochem 13:636–644
Visioli F, Galli C (2001) Phenolics from olive oil and its waste products. Biological activities in vitro and in vivo studies. World Rev Nutr Diet 88:233–237
Visioli F, Bellomo G, Galli C (1998) Free radical-scavenging properties of olive oil polyphenols. Biochem Biophys Res Commun 247:60–64
Visioli F, Galli C, Plasmati E et al (2000) Olive phenol hydroxytyrosol prevents passive smokinginduced oxidative stress. Circulation 102:2169–2171
Wlodek L, Steven HZ (2001) Antioxidants, programmed cell death, and cancer. Nutr Res 21:295–307
Yang DP, Kong DX, Zhang HY (2007) Multiple pharmacological effects of olive oil phenols. Food Chem 104:1269–1271
Zhao B, Yu W, Qian M et al (1997) Involvement of activator protein-1 (AP-1) in induction of apoptosis by vitamin E succinate in human breast cancer cells. Mol Carcinog 19:180–190
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Han, J., Talorete, T.P.N., Yamada, P. et al. Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells. Cytotechnology 59, 45–53 (2009). https://doi.org/10.1007/s10616-009-9191-2
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DOI: https://doi.org/10.1007/s10616-009-9191-2