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Neuroprotective Effect of Fucoidan on H2O2-Induced Apoptosis in PC12 Cells Via Activation of PI3K/Akt Pathway

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Abstract

One of the plausible ways to prevent the reactive oxygen species (ROS)-mediated cellular injury is dietary or pharmaceutical augmentation of endogenous antioxidant defense capacity. In this study, we investigated the neuroprotective effect of fucoidan on H2O2-induced apoptosis in PC12 cells and the possible signaling pathways involved. The results showed that fucoidan inhibited the decrease of cell viability, scavenged ROS formation and reduced lactate dehydrogenase release in H2O2-induced PC12 cells. These changes were associated with an increase in superoxide dismutase and glutathione peroxidase activity, and reduction in malondialdehyde. In addition, fucoidan treatment inhibited apoptosis in H2O2-induced PC12 cells by increasing the Bcl-2/Bax ratio and decreasing active caspase-3 expression, as well as enhancing Akt phosphorylation (p-Akt). However, the protection of fucoidan on cell survival, p-Akt, the Bcl-2/Bax ratio and caspase-3 activity were abolished by pretreating with phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002. In consequence, fucoidan might protect the neurocytes against H2O2-induced apoptosis via reducing ROS levels and activating PI3K/Akt signaling pathway.

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Acknowledgments

This work was supported by the Natural Science Foundation of Shandong Province (No. ZR2011HL068).

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Authors and Affiliations

  1. School of Life Sciences, Yantai University, Yantai, 264005, People’s Republic of China

    Yonglin Gao, Jungang Yin & Jingyu Shen

  2. College of Life Science, Qingdao Agricultural University, Qingdao, 266109, People’s Republic of China

    Chaohua Dong

  3. School of Pharmacy, Yantai University, Yantai, 264005, People’s Republic of China

    Jingwei Tian & Chunmei Li

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  1. Yonglin Gao
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  2. Chaohua Dong
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  3. Jungang Yin
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  4. Jingyu Shen
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  5. Jingwei Tian
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  6. Chunmei Li
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Correspondence to Chunmei Li.

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Gao, Y., Dong, C., Yin, J. et al. Neuroprotective Effect of Fucoidan on H2O2-Induced Apoptosis in PC12 Cells Via Activation of PI3K/Akt Pathway. Cell Mol Neurobiol 32, 523–529 (2012). https://doi.org/10.1007/s10571-011-9792-0

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  • DOI: https://doi.org/10.1007/s10571-011-9792-0

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