Abstract
Background
The purpose of this experimental study was to assess the effects of the immediate postoperative intraperitoneal administration of oxaliplatin and 5-FU on the healing of colonic anastomoses in rats.
Methods
Sixty rats were randomized into 4 groups of 15 rats each and were subjected to colonic anastomoses. To the 1st group, saline solution was administered immediately postoperatively, intraperitoneally. To the 2nd group, 5-FU was administered, to the 3rd group oxaliplatin and to the 4th group 5-FU and oxaliplatin were administered immediately postoperatively, intraperitoneally. After killing the rats on the 8th postoperative day, the anastomoses were examined macroscopically and the anastomotic bursting pressures were measured. The anastomoses were also examined histologically and the hydroxyproline contents were determined.
Results
Rupture of the anastomosis was observed in no rats of the 1st group, in 3 rats of the 2nd group, in 4 rats of the 3rd group and in 7 rats of the 4th group (P = 0.016). The bursting pressure (P < 0.001), the hydroxyproline content (P < 0.001) and the concentration of collagen (P < 0.001) and fibroblasts (P < 0.001) were significantly lower in the 2nd, 3rd and 4th group in comparison with the 1st group. The formation of adhesions and the leukocytosis on the anastomoses were significantly higher in the 2nd, 3rd and 4th group than in the 1st group (P < 0.001).
Conclusions
The immediate postoperative, intraperitoneal administration of oxaliplatin, 5-FU or the combination of 5-FU and oxaliplatin impairs the healing of colonic anastomoses in rats.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kanellos I, Blouhos K, Demetriades H, Pramateftakis MG, Mantzoros I, Zacharakis E, Betsis D (2004) The failed intraperitoneal colon anastomosis after colon resection. Tech Coloproctol 8:53–56
Kanellos I, Odisseos C, Zaramboukas T, Kavouni A, Galovatsea K, Dadoukis I (1997) Colonic healing after early intraperitoneal administration of 5-fluorouracil and interferon in rats. Int J Colorectal Dis 12:45–49
Hribaschek A, Pross M, Kuhn R, Kruger S, Ridwelski K, Halangk W, Boltze C, Lippert H (2002) Prevention and treatment of peritoneal carcinomatosis in experimental investigations with CPT-11 and oxaliplatin. Anti-Cancer Drugs 13:605–614
Sugarbaker PH (2005) Strategies for the prevention and treatment of peritoneal carcinomatosis from gastrointestinal cancer. Cancer Invest 23:155–172
Cihan A, Armutcu F, Ucan BH, Acun Z, Numanoglu VK, Gurel A, Ulukent SC (2003) Comparison of the measurement methods of bursting pressure of intestinal anastomoses. Hepatogastroenterology 50:232–234
Pramateftakis MG, Kanellos D, Demetriades H, Kanellos I, Mantzoros I, Zacharakis E, Despoudi K, Angelopoulos S, Koliakos G, Zaraboukas T, Betsis D (2007) The effects of irinotecan on the healing of colonic anastomoses in rats. Open Surg J 1:1–6
van der Ham AC, Kort WJ, Weijma IM, van den Ingh HF, Jeekel J (1991) Effect of fibrin sealant on the healing colonic anastomosis in the rat. Br J Surg 78:49–53
Phillips JD, Kim CS, Fonkalsrud EW, Zeng H, Dindar H (1992) Effect of chronic corticosteroids and vitamin A on the healing of intestinal anastomoses. Am J Surg 163:71–77
Conflict of interest
The authors certify that they have no conflict of interest related to the publication of this article.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kanellos, D., Pramateftakis, M.G., Mantzoros, I. et al. The effects of the intraperitoneal administration of oxaliplatin and 5-FU on the healing of colonic anastomoses: an experimental study. Tech Coloproctol 15 (Suppl 1), 111–115 (2011). https://doi.org/10.1007/s10151-011-0754-9
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10151-011-0754-9