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D2-40 labeling in lymphangiomyoma/lymphangiomyomatosis of the soft tissue: further evidence of lymphangiogenic tumor histogenesis

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Abstract

We examined ten cases of extrapulmonary lymphangioleiomyoma/lymphangioleiomyomatosis (LAM; all patients female; median age 46.5 years) for immunohistochemical labeling with a monoclonal antibody against podoplanin (D2-40), which is specific for lymphatic endothelial lining. We found positive staining in thin-wall branching vessels reflecting the lymphatic nature of tumor vessels in all cases tested. In contrast, perivascular (HMB-45 positive) myoid cells were not detected by D2-40. The D2-40 labeling confirms the current concept of lymphangiogenic origin of the tumor vessels in LAM. In addition, this study makes a further contribution to the immunohistochemical mapping of this antibody in vascular tumors. Finally, the use of this commercially available antibody provides an additional help in the differential diagnosis of LAM from other soft tissue tumors.

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References

  1. Betlejewski S, Burduk D, Szukalski J (2004) Lymphangioma and lymphangiomyomatosis—the same lesion? Otolaryngol Pol 58:903–906

    PubMed  Google Scholar 

  2. Bonsib S (1996) HMB-45 reactivity in renal leiomyomas and leiomyosarcomas. Mod Path 9:664–669

    CAS  Google Scholar 

  3. Chen SS (1989) Propensity of retroperitoneal lymph node metastasis in patients with stage I sarcoma of the uterus. Gynecol Oncol 32:215–217

    Article  PubMed  CAS  Google Scholar 

  4. Debelenko LV, Perez-Atayde AR, Mulliken JB, Liang MG, Archibald TH, Kozakevich HP (2005) D2-40 immunohistochemical analysis of pediatric vascular tumors reveals positivity in kaposiform hemangioendothelioma. Mod Path 18:1454–1460

    Article  Google Scholar 

  5. Fiore MG, Sanguedolce F, Lolli I, Piscitelli D, Ricco R (2005) Abdominal lymphangioleiomyomatosis in a man with Klinefelter syndrome: the first reported case. Ann Diagn Pathol 9:96–100

    Article  PubMed  Google Scholar 

  6. Folpe AL (2002) Neoplasms with perivascular epithelioid cell differentiation (PEComas). In: Fletcher CDM, Unni KK, Mertens F (eds) WHO classification of tumours of soft tissue and bone. IARC, Lyon, pp 221–222

    Google Scholar 

  7. Franke FE, Steger K, Marks A, Kutzner H, Mentzel T (2004) Hobnail hemangiomas (targetoid hemosiderotic hemangiomas) are true lymphangiomas. J Cutan Pathol 31:362–367

    Article  PubMed  Google Scholar 

  8. Fukunaga M (2005) Expression of D2-40 in lymphatic endothelium of normal tissues and in vascular tumors. Histopathology 46:396–402

    Article  PubMed  CAS  Google Scholar 

  9. Hansen T, Katenkamp K, Katenkamp D (2006) D2-40 staining in sinonasal-type hemangiopericytoma—further evidence of distinction from conventional hemangiopericytoma and solitary fibrous tumor. Virchows Arch 448:459–462

    Article  PubMed  CAS  Google Scholar 

  10. Hornick JL, Fletcher CDM (2006) PEComa: what do we know so far? Histopathology 48:75–82

    Article  PubMed  CAS  Google Scholar 

  11. Kahn HJ, Bailey D, Marks A (2002) Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi’s sarcoma and a subset of angiosarcomas. Mod Path 15:434–440

    Article  Google Scholar 

  12. Kaiserling E (2004) Immunohistochemical identification of lymph vessels with D2-40 in diagnostic pathology. Pathologe 25:362–374

    Article  PubMed  CAS  Google Scholar 

  13. Kumasaka T, Seyama K, Mitani K, Sato T, Souma S, Kondo T, Hayashi S, Minami M, Uekusa T, Fukuchi Y, Suda K (2004) Lymphangiogenesis in lymphangioleiomyomatosis. Its implication in the progression of lymphangioleiomyomatsosis. Am J Surg Pathol 28:1007–1016

    Article  PubMed  Google Scholar 

  14. Leitao MM, Sonoda Y, Brennan MF, Barakat RR, Chi DS (2003) Incidence of lymph node and ovarian metastases in leiomyosarcoma of the uterus. Gynecol Oncol 91:209–212

    Article  PubMed  Google Scholar 

  15. Marks A, Sutherland DR, Bailey D, Iglesias J, Law J, Lei M, Yeger H, Banerjee D, Baumal R (1999) Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumors. Br J Cancer 80:569–578

    Article  PubMed  CAS  Google Scholar 

  16. Matsui K, Tatsuguchi A, Valencia J, Yu ZX, Bechtle J, Beasley MB, Avila N, Travis WD, Moss J, Ferrans VJ (2000) Extrapulmonary lymphangioleiomyomatosis (LAM): clinicopathologic features in 22 cases. Human Pathol 31:1242–1248

    Article  CAS  Google Scholar 

  17. Miettinen M (ed) (2003) Diagnostic soft tissue pathology. Churchill Livingstone, Philadelphia, pp 248–249

    Google Scholar 

  18. Ordonez NG (2006) Podoplanin: a novel diagnostic immunohistochemical marker. Adv Anat Pathol 13:83–88

    Article  PubMed  CAS  Google Scholar 

  19. Pusztaszeri MP, Seelentag W, Bosman FT (2006) Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and fli-1 in normal human tissues. J Histochem Cytochem 54:385–395

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We thank Mrs. Bergholz, Mrs. Dietrich, and Mrs. Pelzer (Institute of Pathology, Jena) as well as Mrs. Roth (Institute of Pathology, Mainz) for the excellent technical assistance.

We are very grateful to all pathologists who sent case material and clinical details from patients included in this study.

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Correspondence to Torsten Hansen.

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Hansen, T., Katenkamp, K., Bittinger, F. et al. D2-40 labeling in lymphangiomyoma/lymphangiomyomatosis of the soft tissue: further evidence of lymphangiogenic tumor histogenesis. Virchows Arch 450, 449–453 (2007). https://doi.org/10.1007/s00428-007-0376-9

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  • DOI: https://doi.org/10.1007/s00428-007-0376-9

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