Abstract
A method is presented to conjugate horseradish peroxidase (HRP) to oligodeoxynucleotides for fluorescence in situ hybridization assays employing tyramide signal amplification (TSA). HRP is covalently bound to the oligonucleotide by thiol ether linkage and purified by high-performance liquid chromatography. With TSA detection, a single HRP-labeled oligonucleotide probe is sufficient for in situ detection of clustered DNA repeat sequences with a degree of repetition between 20 and 50.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Accepted: 6 December 1999
Rights and permissions
About this article
Cite this article
van Gijlswijk, R., van de Corput, M., Bezrookove, V. et al. Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization. Histochemistry 113, 175–180 (2000). https://doi.org/10.1007/s004180050436
Issue Date:
DOI: https://doi.org/10.1007/s004180050436