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Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization

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Histochemistry and Cell Biology Aims and scope Submit manuscript

Abstract 

A method is presented to conjugate horseradish peroxidase (HRP) to oligodeoxynucleotides for fluorescence in situ hybridization assays employing tyramide signal amplification (TSA). HRP is covalently bound to the oligonucleotide by thiol ether linkage and purified by high-performance liquid chromatography. With TSA detection, a single HRP-labeled oligonucleotide probe is sufficient for in situ detection of clustered DNA repeat sequences with a degree of repetition between 20 and 50.

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Accepted: 6 December 1999

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van Gijlswijk, R., van de Corput, M., Bezrookove, V. et al. Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization. Histochemistry 113, 175–180 (2000). https://doi.org/10.1007/s004180050436

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  • DOI: https://doi.org/10.1007/s004180050436

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