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Expression of transforming growth factor β in renal cell carcinoma and matched non-involved renal tissue

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Abstract

TGFβ1 is one of several cytokines produced by proximal tubular and renal cancer cells. Previous studies have been mainly focused on determining plasma or serum TGFβ levels, its effect on RCC cultures, and the expression of TGFβ mRNA. Cancerous and autologous normal kidney samples were obtained from 24 patients treated by radical nephrectomy. TGFβ1 expression was determined using a semi quantitative Western blot analysis and immunohistochemistry. Blot densities and immunohistochemical expression intensities in normal and neoplastic tissue were compared, and subsequently correlated to tumor stage, histological type and nuclear grade. All tissue samples examined expressed TGFβ1; mean tumor to non-involved kidney spot density ratio correlated with advancing stage and higher nuclear grade. The overexpression of TGFβ1 in certain RCCs may partially explain their resistance to the growth suppression action of TGFβ. The correlation with tumor stage and grade indicates a possible role in the development of metastatic potential as well as in host’s immune response modulation.

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Acknowledgements

The authors acknowledge the skillful technical contribution of Penelope Chiara (medical arts), Despina Karandrea and Vasiliki Dotsika.

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Authors and Affiliations

  1. Department of Urology, University of Athens Medical School, 75, Mikras Asias St., 115-27, Athens, Greece

    Dionisios Mitropoulos, Efraim Serafetinidis, Anastasios Zervas, Ioannis Anastasiou & Constantinos Dimopoulos

  2. Department of Histology and Embryology, University of Athens Medical, School, Athens, Greece

    Aspasia Kiroudi

  3. Department of Experimental Surgery and Surgical Research, University of Athens Medical School, Athens, Greece

    Evangelia Christelli

Authors
  1. Dionisios Mitropoulos
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  2. Aspasia Kiroudi
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  3. Evangelia Christelli
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  4. Efraim Serafetinidis
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  5. Anastasios Zervas
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  6. Ioannis Anastasiou
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  7. Constantinos Dimopoulos
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Correspondence to Dionisios Mitropoulos.

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Mitropoulos, D., Kiroudi, A., Christelli, E. et al. Expression of transforming growth factor β in renal cell carcinoma and matched non-involved renal tissue. Urol Res 32, 317–322 (2004). https://doi.org/10.1007/s00240-003-0360-z

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  • DOI: https://doi.org/10.1007/s00240-003-0360-z

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