Summary
The expression of keratins 8 and 14 was investigated immunohistochemically by the avidin-biotin-peroxidase (ABC) method using formalin-fixed paraffinembedded specimens from 42 tumours of human skin appendages. Results were compared with the staining of 34 specimens from normal skin and skin appendages adjacent to the tumours. Keratin 14 was detected by the monoclonal antibody (mAb) 312C8-1, and was found in the basal cells of the epidermis, the outer root sheaths of hair follicles, and the peripheral cells of sebaceous glands. It was also detected in the inner and outer layers of cells in the ductal portion and the myoepithelial cells in the secretory portion of apocrine and eccrine sweat glands. Keratin 8 was detected by mAb 35BH11, and was present in the secretory cells of eccrine and apocrine sweat glands but not in myoepithelial or ductal cells. The pilosebaceous apparatus and the epidermis were uniformly negative. In benign skin appendage tumours, the staining patterns for both keratins generally resembled their distribution in the corresponding normal tissues. The demonstration of keratins 8 and 14 may be useful in the recognition, classification and diagnosis of skin appendage tumours.
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References
Battifora H, Kopinski A (1986) The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J Histochem Cytochem 34:1095–1100
Cooper D, Schermer A, Sun T-T (1985) Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations. Lab Invest 52:243–256
Dairkee SH, Blayney C, Smith HS, Hackett AJ (1985) Monoclonal antibody that defines human myoepithelium. Proc Natl Acad Sci USA 82:7409–7413
Gown AM, Vogel AM (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am J Pathol 114:309–321
Hashimoto K, Lever WF (1964) Ecrine poroma; histochemical and electron microscopic studies. J Invest Dermatol 43:237–247
Hashimoto K, Mehregan AH, Kumakiri M (1987) Tumors of skin appendages. Practical dermatopathology series. Butterworths, Boston
Lever WF, Schaumburg-Lever G (1983) Histopathology of the skin, 6th edn. Lippincott, Philadelphia
Mazoujian G, Margolis R (1988) Immunohistochemistry of gross cystic disease fluid protein (GCDFP-15) in 65 benign sweat gland tumors of the skin. Am J Dermatopathol 10:28–35
Menard S, Tagliabeu E, Canevari S, Fossati G, Colnaghi MI (1983) Generation of monoclonal antibodies reacting with normal and cancer cells of human breast. Cancer Res 43:1295–1300
Moll R, Franke WW, Schiller DL, Geiger B, Krepier R (1982a) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Franke WW, Volc-Platzer B, Krepier R (1982b) Different keratin polypeptides in epidermis and other epithelia of human skin: a specific cytokeratin of molecular weight 46,000 in epithelia of the pilosebaceous tract and basal cell epitheliomas. J Cell Biol 95:285–295
Noda Y, Kumasa S, Higashiyama H, Mori M (1988) Immunolocalization of keratin proteins in sweat gland tumours by the use of monoclonal antibody. Pathol Res Pract 183:284–291
Penneys NS, Nadji M, McKinney EC (1981) Carcinoembryonic antigen is present in human eccrine sweat. J Am Acad Dermatol 4:401–403
Penneys NS, Nadji M, Morales A (1982) Carcinoembryonic antigen in benign sweat gland tumors. Arch Dermatol 118:225–227
Pinkus GS, O'Connor EM, Etheridge CL, Corson JM (1985) Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumors using polyclonal and monoclonal antibodies. J Histochem Cytochem 33:465–473
Pinkus H, Iwasaki T, Mishima Y (1981) Outer root sheath keratinization in anagen and catagen of the mammalian hair follicle. A seventh distinct type of keratinization in the hair follicle: trichilemmal keratinization. J Anat 133:19–35
Tappeiner J, Wolfe K (1969) Hidradenoma papilliferum. Eine enzymhistochemische und electronenmikroskopische Studie. Hautarzt 19:101–109
Tsubura A, Morii S, Ueda S, Sasaki M, Zotter S, Watzig V, Mooi W, Hageman PC, Hilkens J, Van den Tweel J, Meijer C, Hilgers J (1987) Immunohistochemical demonstration of MAM-3 and MAM-6 antigens in normal human skin appendages and their tumors. Arch Dermatol Res 279:550–557
Tsubura A, Shikata N, Inui T, Morii S, Hatano T, Oikawa T, Matsuzawa A (1988) Immunohistochemical localization of myoepithelial cells and basement membrane in normal, benign and malignant breast lesions. Virchows Arch [A] 413:133–139
Zuk JA, West KP, Fletcher A (1988) Immunohistochemical staining patterns of sweat glands and their neoplasms using two monoclonal antibodies to keratins. J Cutan Pathol 15:8–17
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Tsubura, A., Okada, H., Sasaki, M. et al. Immunohistochemical demonstration of keratins 8 and 14 in benign tumours of the skin appendage. Vichows Archiv A Pathol Anat 418, 503–507 (1991). https://doi.org/10.1007/BF01606500
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DOI: https://doi.org/10.1007/BF01606500