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Visualizing Immunity pp 153–177Cite as

Visualizing the Molecular and Cellular Events Underlying the Initiation of B-Cell Activation

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 334))

Abstract

The appropriate activation of B cells is critical for the development of effective immune responses. B cell activation is initiated following the engagement of the B cell receptor (BCR) with specific antigen. The spatiotemporal characterization of the ensuing molecular and cellular events has been the subject of recent high-resolution imaging investigations. In this review we highlight information gathered thus far concerning the initial processes underlying the activation of B cells. First, we consider studies that have offered new insights into the early molecular events that occur within the B cell prior to formation of the immunological synapse. As such, BCR-microclusters formed on engagement with antigen have been identified as the sites of active signaling and assembly of “microsignalosomes.” Furthermore, signaling through these “microsignalosomes” is propagated and enhanced through B cell spreading in response to membrane-antigen in a CD19-dependent manner. Finally, we discuss a number of multiphoton microscopy studies that have enabled dynamic characterization of the initial encounters between B cells and antigen in vivo. These investigations visualize the presentation of larger antigens to B cells via cell-mediated strategies, involving macrophages in the subcapsular sinus and dendritic cells in the paracortex.

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Acknowledgments

We would like to thank members of the Lymphocyte Interaction Laboratory for helpful discussions and critical reading of the manuscript.

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  1. Lymphocyte Interaction Laboratory, Cancer Research UK London Research Institute, Lincoln’s Inn Fields Laboratories, 44 Lincoln’s Inn Fields, London, WC2A 3PX, UK,

    Naomi E. Harwood & Facundo D. Batista

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  1. Naomi E. Harwood
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  2. Facundo D. Batista
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Correspondence to Facundo D. Batista .

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    © 2009 Springer-Verlag Berlin Heidelberg

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    Harwood, N.E., Batista, F.D. (2009). Visualizing the Molecular and Cellular Events Underlying the Initiation of B-Cell Activation. In: Dustin, M., McGavern, D. (eds) Visualizing Immunity. Current Topics in Microbiology and Immunology, vol 334. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-93864-4_7

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