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Cancer Stem Cells pp 43–48Cite as

Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1692))

Abstract

The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids. In this chapter, it is described the role of ALDH in the identification of cancer stem cells (CSCs), having been shown that stem cells express high levels of ALDH. Here, we present a method called ALDEFLUOR assay used for the identification, evaluation, and isolation of normal, cancer stem and progenitor cells.

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Author information

Authors and Affiliations

  1. Department of Experimental Medicine, Medical School, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Luigi Mele, Davide Liccardo & Virginia Tirino

Authors
  1. Luigi Mele
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  2. Davide Liccardo
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  3. Virginia Tirino
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Corresponding author

Correspondence to Virginia Tirino .

Editor information

Editors and Affiliations

  1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

  2. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Vincenzo Desiderio

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Mele, L., Liccardo, D., Tirino, V. (2018). Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay. In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7401-6_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7400-9

  • Online ISBN: 978-1-4939-7401-6

  • eBook Packages: Springer Protocols

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