Regular ArticleFluorimetric Detection of Aldehyde Dehydrogenase Activity in Human Blood, Saliva, and Organ Biopsies and Kinetic Differentiation between Class I and Class III Isozymes
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Optical substrates for drug-metabolizing enzymes: Recent advances and future perspectives
2022, Acta Pharmaceutica Sinica BCitation Excerpt :The sensitivity of the proposed ALDH assay is sufficient to measure the catalytic activity of the enzyme in diluted blood lysates without any pre-purification by a continuous method. Later, they found similar structure 6-methoxy-2-naphthaldehyde was selective oxidized by class III ALDH present in human saliva60. The currently available commercial assay for identifying ALDH bright cells (cells with high ALDH activity) is based on enzymatic hydrolysis of a fluorescent substrate ALDEFLUOR (ALDHs-F2) to produce green fluorescence61.
The alterations in alcohol dehydrogenase and aldehyde dehydrogenase activities in the sera of patients with renal cell carcinoma
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2017, International Journal of Biological MacromoleculesThe diagnostic significance of serum alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in renal cell cancer patients
2016, Experimental and Molecular PathologyThe activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in renal cell carcinoma
2015, Experimental and Molecular PathologyCitation Excerpt :The reduction of NDMA was monitored at 440 nm on a Shimadzu UV/VIS 1202 spectrophotometer (Shimadzu Europa GmbH, Duisburg, Germany). Aldehyde dehydrogenase activity was measured using the fluorogenic method based on the oxidation of 6-methoxy-2-napthaldehyde to fluorescent 6-methoxy-2-naphthoate (Jelski et al., 2007, 2009; Wierzchowski et al., 1997). The reaction mixture contained 60 μL of supernatant, 60 μL of substrate, 20 μL of 11.4 mM NAD and 2.8 mL of 50 mM of sodium phosphate buffer, pH 8.5.
The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in ovarian cancer and ovarian cysts
2013, Advances in Medical SciencesCitation Excerpt :The reduction of NDMA was monitored at 440 nm on a Shimadzu UV/VIS 1202 spectrophotometer (Shimadzu Europa GmbH, Duisburg, Germany). Aldehyde dehydrogenase activity was measured using the fluorogenic method based on the oxidation of 6-methoxy-2-naphtaldehyde to fluorescent 6-methoxy-2-naphtoate [11]. The reaction mixture contained 60 μL of supernatant, 60 μL of substrate, 20 μL of 11.4 mM NAD and 2.8 mL of 50 mM of sodium phosphate buffer, pH 8.5.