RT Journal Article SR Electronic T1 Methylation of the TFPI2 Gene Is Frequently Detected in Advanced Gastric Carcinoma JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4131 OP 4133 VO 30 IS 10 A1 HIBI, KENJI A1 GOTO, TETSUHIRO A1 KITAMURA, YO-HEI A1 SAKURABA, KAZUMA A1 SHIRAHATA, ATSUSHI A1 MIZUKAMI, HIROKI A1 SAITO, MITSUO A1 ISHIBASHI, KAZUYOSHI A1 KIGAWA, GAKU A1 NEMOTO, HIROSHI A1 SANADA, YUTAKA YR 2010 UL http://ar.iiarjournals.org/content/30/10/4131.abstract AB Background: Recently, Glockner et al. identified the methylation of TFPI2 as a frequent event in human colorectal cancer using a gene expression array-based strategy. Materials and Methods: Methylation status of the TFPI2 gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results: Aberrant methylation of the TFPI2 gene was detected in 7 out of 38 (18%) primary gastric carcinomas, suggesting that the methylation of TFPI2 is frequently observed in gastric carcinomas. The clinicopathological data were correlated with the methylation results. A significant difference was observed in maximal tumour size (p=0.0084), extent of tumour (p=0.0068), and TNM stage (p=0.0392). Conclusion: TFPI2 is frequently methylated in advanced gastric carcinomas.