PT - JOURNAL ARTICLE AU - HIBI, KENJI AU - GOTO, TETSUHIRO AU - KITAMURA, YO-HEI AU - SAKURABA, KAZUMA AU - SHIRAHATA, ATSUSHI AU - MIZUKAMI, HIROKI AU - SAITO, MITSUO AU - ISHIBASHI, KAZUYOSHI AU - KIGAWA, GAKU AU - NEMOTO, HIROSHI AU - SANADA, YUTAKA TI - Methylation of the <em>TFPI2</em> Gene Is Frequently Detected in Advanced Gastric Carcinoma DP - 2010 Oct 01 TA - Anticancer Research PG - 4131--4133 VI - 30 IP - 10 4099 - http://ar.iiarjournals.org/content/30/10/4131.short 4100 - http://ar.iiarjournals.org/content/30/10/4131.full SO - Anticancer Res2010 Oct 01; 30 AB - Background: Recently, Glockner et al. identified the methylation of TFPI2 as a frequent event in human colorectal cancer using a gene expression array-based strategy. Materials and Methods: Methylation status of the TFPI2 gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results: Aberrant methylation of the TFPI2 gene was detected in 7 out of 38 (18%) primary gastric carcinomas, suggesting that the methylation of TFPI2 is frequently observed in gastric carcinomas. The clinicopathological data were correlated with the methylation results. A significant difference was observed in maximal tumour size (p=0.0084), extent of tumour (p=0.0068), and TNM stage (p=0.0392). Conclusion: TFPI2 is frequently methylated in advanced gastric carcinomas.