TY - JOUR T1 - Sodium Butyrate with UCN-01 Has Marked Antitumour Activity against Cervical Cancer Cells JF - Anticancer Research JO - Anticancer Res SP - 4049 LP - 4061 VL - 30 IS - 10 AU - ANNE-ZÉLIE DECRION-BARTHOD AU - MATTHIEU BOSSET AU - MARIE-LAURE PLISSONNIER AU - ANTONIO MARCHINI AU - MAGALIE NICOLIER AU - SOPHIE LAUNAY AU - JEAN-LUC PRÉTET AU - JEAN ROMMELAERE AU - CHRISTIANE MOUGIN Y1 - 2010/10/01 UR - http://ar.iiarjournals.org/content/30/10/4049.abstract N2 - Aim: The effect of combining sodium butyrate (NaB), a histone deacetylase inhibitor, and 7-hydroxy-staurosporine (UCN-01) on cytotoxicity in human cervical carcinoma cells was evaluated. Materials and Methods: HeLa and CaSki cells were treated using NaB alone or in combination with staurosporine (STS) or its analog UCN-01. Cytotoxicity was determined by flow cytometry and morphological assays. Apoptotic pathways were characterized by Western blotting and immunostaining. CaSki cells were also xenografted into nude mice to assess the in vivo effects of NaB/UCN-01 combination. Results: Treatment with NaB and STS or UCN-01 resulted in enhanced apoptosis of cancer cells. Apoptosis involved mitochondrial pathways and overexpression of p53 and p73. In concordance, co-treatment modulated some p53/p73 downstream targets such as p21, BAX, BCL-2 and BCL-XL, leading to increased caspase-3 and poly(ADP-ribose) polymerase cleavage. In vivo, NaB/UCN-01 combination exerted a substantial tumour growth suppression effect compared with single treatment. Conclusion: UCN-01 was shown to be a potentiator of NaB therapy for cervical cancer cells. ER -