TY - JOUR T1 - Expression of 25-Hydroxyvitamin D<sub>3</sub>-24-Hydroxylase in Benign and Malignant Ovarian Cell Lines and Tissue JF - Anticancer Research JO - Anticancer Res SP - 3635 LP - 3639 VL - 29 IS - 9 AU - DOROTHEA FISCHER AU - MARLENE THOMÉ AU - STEFFI BECKER AU - TIM CORDES AU - KLAUS DIEDRICH AU - MICHAEL FRIEDRICH AU - MARC THILL Y1 - 2009/09/01 UR - http://ar.iiarjournals.org/content/29/9/3635.abstract N2 - Calcitriol (1,25-dihydroxyvitamin D3, 1,25(OH)2D3) plays a pivotal role in maintaining calcium and phosphate homeostasis. Aside from that, it supports the native and attenuates the acquired immune system and has positive effects on cell growth, differentiation and the prevention of carcinogenesis. The goal of this study was to detect possible differences in the expression of the calcitriol-degrading enzyme 24-hydroxylase (24-OHase) between malignant and benign ovarian cell lines and tissue. The analyses were based on real-time PCR, nested touchdown PCR and Western blot. When compared to benign granulose GLZ cells, the malignant HGL5 cells showed a significantly higher 24-OHase expression at the protein level (p&lt;0.01). In the malignant ovarian tissue, the expression was significantly higher in RNA (p&lt;0.001), but lower at the protein level (p&lt;0.01). An increased 24-OHase expression could indicate a decrease in available calcitriol. ER -