TY - JOUR T1 - 25-Hydroxyvitamin D<sub>3</sub> 1α-Hydroxylase Splice Variants in Benign and Malignant Ovarian Cell Lines and Tissue JF - Anticancer Research JO - Anticancer Res SP - 3627 LP - 3633 VL - 29 IS - 9 AU - DOROTHEA FISCHER AU - MARLENE THOMÉ AU - STEFFI BECKER AU - TIM CORDES AU - KLAUS DIEDRICH AU - MICHAEL FRIEDRICH AU - MARC THILL Y1 - 2009/09/01 UR - http://ar.iiarjournals.org/content/29/9/3627.abstract N2 - Calcitriol is judged to have a positive effect on control of the immune system, cell growth and differentiation and therefore, the prevention of cancer genesis. The aim of this study was to detect any possible differences in the 25-hydroxyvitamin D-1α-hydroxylase (1αOHase)-expression between benign and malignant ovarian tissue and cell lines. The analysis was conducted quantitatively, by means of nested “touchdown” PCR and Western blot, and qualitatively, with the use of real-time PCR and Western blot. The gene structure was sequenced. Compared to the benign cell line, the malignant cell lines showed a significantly higher expression of 1αOHase at the RNA level. A statistically lower expression of the 1αOHase protein was found in the malignant tissue. In the malignant cell lines and tissues, divergent bands were detected, which led to various splice variants on sequencing. Their increased expression in malignancy is possibly bound to the reduction of enzyme activity, which may lead to the genesis of ovarian cancer. In the future, preventive and therapeutic activities may result from these findings. ER -