PT  - JOURNAL ARTICLE
AU  - TAKAHIRO YOSHIDA
AU  - TAKANORI MIYOSHI
AU  - JUN-ICHI SEIKE
AU  - HIROMICHI YAMAI
AU  - HIROKAZU TAKECHI
AU  - YASUHIRO YUASA
AU  - AKIRA TANGOKU
TI  - Gene Expression Changes in a Chemoresistant Model with Human Esophageal Cancer Xenografts Using cDNA Microarray
DP  - 2009 Apr 01
TA  - Anticancer Research
PG  - 1163--1168
VI  - 29
IP  - 4
4099  - http://ar.iiarjournals.org/content/29/4/1163.short
4100  - http://ar.iiarjournals.org/content/29/4/1163.full
SO  - Anticancer Res2009 Apr 01; 29
AB  - Background: 5-Fluorouracil (5-FU) and cisplatin combined chemotherapy (FP) is commonly used for esophageal cancer. Acquired resistance needs to be overcome to improve the chemotherapeutic effect. Materials and Methods: The FP-resistant xenograft model using severe combined immunodeficient (SCID) mice was established as an acquired resistance model. RNA was extracted pretreatment, at the onset of the anticancer effect, during the most effective, and regrowth period in the FP administration group and during the mid-progressive period and the far advanced period in the control group. A microarray was applied to explore gene expression changes. Results: The data set containing up-regulated genes in the regrowth period was uploaded into Ingenuity Pathway Analysis. The expression change profiles suggested that activation of not only 5-FU- and cisplatin-specific genes, but also the Phosphoinositide 3-kinase (PI3K)/AKT signal were associated with FP resistance. Conclusion: A xenograft model using SCID mice with esophageal cancer cells would monitor gene changes during treatment and regrowth.