RT Journal Article
SR Electronic
T1 3-Dimensional Microarray Analysis of Estrogen Signal-related Genes in Breast Cancer Tissues
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3971
OP 3975
VO 29
IS 10
A1 MITSUYO MATSUMOTO
A1 HIROKO SAKAMOTO
A1 YURI YAMAGUCHI
A1 YUKO SEINO
A1 HIROYUKI TAKEI
A1 MASAFUMI KUROSUMI
A1 HIRONOBU SASANO
A1 NOBUO YAEGASHI
A1 SHIN-ICHI HAYASHI
YR 2009
UL http://ar.iiarjournals.org/content/29/10/3971.abstract
AB Background: Expression analysis of estrogen response genes (ERGs) may help to predict the effectiveness of endocrine therapies in breast cancer patients. We produced a custom-made, 3-dimensional microarray system (3DMS), using previously identified ERGs, and analyzed expression of ERGs on breast cancer tissues. Materials and Methods: aRNA was synthesized from 27 breast cancer tumors. The aRNAs were applied to the infrastructure of a 3DMS with spotted cDNA probes to 36 ERGs. Data were analyzed by cluster analysis. Results: All 27 specimens were classified clearly into one of two clusters based on whether the ERGs were up or down regulated. These groups correlated with high expression of ER (P<0.05) and Her2 (P<0.05). In some cases, ERG expressions were low even though the tumor was ER positive. Conclusion: 3DMS may be useful for accurately predicting prognosis and whether endocrine therapies targeting the ER would be effective in an individual breast cancer patient.