RT Journal Article
SR Electronic
T1 Hematogenous Cytokeratin 20 mRNA Detection Has Prognostic Impact in Oral Squamous Cell Carcinoma: Preliminary Results
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 291
OP 297
VO 29
IS 1
A1 TAKESHI TOYOSHIMA
A1 ELEFTHERIOS VAIRAKTARIS
A1 EMEKA NKENKE
A1 KARL ANDREAS SCHLEGEL
A1 FRIEDRICH WILHELM NEUKAM
A1 JUTTA RIES
YR 2009
UL http://ar.iiarjournals.org/content/29/1/291.abstract
AB Background: The study was designed to detect disseminated tumor cells (DTCs) in postoperative peripheral blood of patients with oral squamous cell carcinoma (OSCC) and to determine their relevance as prognostic markers by cytokeratin (CK) expression analysis. Materials and Methods: Forty samples of peripheral blood mononuclear cells (PBMCs) isolated 4 weeks after surgery were screened for occurrence of four CK mRNA transcripts by real-time quantitative RT qPCR. Detection of mRNA expression was compared with clinicopathological parameters and disease-free survival (DFS). Results: CK 17 and CK 19 could not be detected in any samples. CK 18 and CK 20 were detectable in 1 (2.5%) and in 14 (35.0%), respectively. The detection of CK 20 was not significantly associated with lymph node status, clinical stage, or differentiation grade, but was significantly higher in patients with T3 and T4 OSCC (p=0.04). DFS was not associated with tumor size, clinical stage, or differentiation grade. But poor DFS was significantly associated with the occurrence of lymph node metastasis (p=0.01) and detection of CK 20 (p=0.01). Conclusion: DTCs in PBMCs of postoperative patients with OSCC could only be detected by determination of CK 20 mRNA. Detection of CK 20 mRNA in peripheral blood seems to be of relevance for prognosis in OSCC. Copyright© 2009 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved