TY - JOUR T1 - Effects of Cold Atmospheric Plasma on the Expression of Chemokines, Growth Factors, TNF Superfamily Members, Interleukins, and Cytokines in Human Osteosarcoma Cells JF - Anticancer Research JO - Anticancer Res SP - 151 LP - 157 DO - 10.21873/anticanres.13091 VL - 39 IS - 1 AU - LYUBOMIR HARALAMBIEV AU - LASSE WIEN AU - NADINE GELBRICH AU - AXEL KRAMER AU - ALEXANDER MUSTEA AU - MARTIN BURCHARDT AU - AXEL EKKERNKAMP AU - MATTHIAS B. STOPE AU - DENIS GÜMBEL Y1 - 2019/01/01 UR - http://ar.iiarjournals.org/content/39/1/151.abstract N2 - Background/Aim: Therapeutic options for osteosarcoma (OS) are still limited. Cold atmospheric plasma (CAP) leads to inhibition of tumor growth and metastasis, but underlying mechanisms are not fully understood. The aim of this study was to investigate CAP-induced changes in cytokine expression in OS cells. Materials and Methods: OS cell lines (U2-OS, MNNG/HOS) were treated with CAP and administered to an RT2 Profiler PCR Array (Qiagen, Hilden, Germany) detecting 84 chemokines, growth factors, TNF superfamily members, interleukins, and cytokines. Results: The analyses showed that 15 factors (C5, CCL5, CNTF, CSF1, CSF3, CXCL1, IL-1A, IL-1B, IL-18, IL-22, IL23A, MSTN, NODAL, TGFβ2, THPO) were induced, but only one factor (VEGFA) was suppressed after CAP treatment. Conclusion: No extensive systemic cell response with presumably far-reaching consequences for neighboring cells was detectable after CAP treatment. Since the antitumoral effect of CAP on OS cells has already been demonstrated, intraoperative treatment with CAP represents a promising and systemic safe option for the therapy of OS. ER -