RT Journal Article SR Electronic T1 Infrequent Amplification of JUN in Hepatocellular Carcinoma JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4989 OP 4994 VO 29 IS 12 A1 MIO ENDO A1 KOHICHIROH YASUI A1 TOMOAKI NAKAJIMA A1 YASUYUKI GEN A1 KAZUHIRO TSUJI A1 OSAMU DOHI A1 KEIKA ZEN A1 HIRONORI MITSUYOSHI A1 MASAHITO MINAMI A1 YOSHITO ITOH A1 MASAFUMI TANIWAKI A1 SHINJI TANAKA A1 SHIGEKI ARII A1 TAKESHI OKANOUE A1 TOSHIKAZU YOSHIKAWA YR 2009 UL http://ar.iiarjournals.org/content/29/12/4989.abstract AB Aim: To determine whether JUN (the oncogene encoding c-Jun protein) is amplified and overexpressed in hepatocellular carcinoma (HCC). Materials and Methods: DNA copy number aberrations were investigated using a high-density oligonucleotide microarray. DNA copy numbers were determined by fluorescence in situ hybridization. Genomic DNA and mRNA were quantified using real-time quantitative PCR. Results: A novel amplification was found at the chromosomal region 1p32-31 in a JHH-2 HCC cell line within which JUN is amplified and overexpressed. However, no copy number gain of JUN (>2-fold) was observed in 34 primary HCC tumors. Rather, a loss of JUN (<0.5-fold) was seen in 13 (38%) out of the 34 tumors and expression of JUN was significantly lower in 26 (70%) out of the 37 HCC tumors compared with their nontumorous counterparts. Conclusion: Although JUN was amplified and overexpressed in JHH-2 HCC cells, amplification and overexpression of JUN may be rare in primary HCCs.