PT - JOURNAL ARTICLE AU - MIO ENDO AU - KOHICHIROH YASUI AU - TOMOAKI NAKAJIMA AU - YASUYUKI GEN AU - KAZUHIRO TSUJI AU - OSAMU DOHI AU - KEIKA ZEN AU - HIRONORI MITSUYOSHI AU - MASAHITO MINAMI AU - YOSHITO ITOH AU - MASAFUMI TANIWAKI AU - SHINJI TANAKA AU - SHIGEKI ARII AU - TAKESHI OKANOUE AU - TOSHIKAZU YOSHIKAWA TI - Infrequent Amplification of <em>JUN</em> in Hepatocellular Carcinoma DP - 2009 Dec 01 TA - Anticancer Research PG - 4989--4994 VI - 29 IP - 12 4099 - http://ar.iiarjournals.org/content/29/12/4989.short 4100 - http://ar.iiarjournals.org/content/29/12/4989.full SO - Anticancer Res2009 Dec 01; 29 AB - Aim: To determine whether JUN (the oncogene encoding c-Jun protein) is amplified and overexpressed in hepatocellular carcinoma (HCC). Materials and Methods: DNA copy number aberrations were investigated using a high-density oligonucleotide microarray. DNA copy numbers were determined by fluorescence in situ hybridization. Genomic DNA and mRNA were quantified using real-time quantitative PCR. Results: A novel amplification was found at the chromosomal region 1p32-31 in a JHH-2 HCC cell line within which JUN is amplified and overexpressed. However, no copy number gain of JUN (&gt;2-fold) was observed in 34 primary HCC tumors. Rather, a loss of JUN (&lt;0.5-fold) was seen in 13 (38%) out of the 34 tumors and expression of JUN was significantly lower in 26 (70%) out of the 37 HCC tumors compared with their nontumorous counterparts. Conclusion: Although JUN was amplified and overexpressed in JHH-2 HCC cells, amplification and overexpression of JUN may be rare in primary HCCs.