%0 Journal Article %A MIO ENDO %A KOHICHIROH YASUI %A TOMOAKI NAKAJIMA %A YASUYUKI GEN %A KAZUHIRO TSUJI %A OSAMU DOHI %A KEIKA ZEN %A HIRONORI MITSUYOSHI %A MASAHITO MINAMI %A YOSHITO ITOH %A MASAFUMI TANIWAKI %A SHINJI TANAKA %A SHIGEKI ARII %A TAKESHI OKANOUE %A TOSHIKAZU YOSHIKAWA %T Infrequent Amplification of JUN in Hepatocellular Carcinoma %D 2009 %J Anticancer Research %P 4989-4994 %V 29 %N 12 %X Aim: To determine whether JUN (the oncogene encoding c-Jun protein) is amplified and overexpressed in hepatocellular carcinoma (HCC). Materials and Methods: DNA copy number aberrations were investigated using a high-density oligonucleotide microarray. DNA copy numbers were determined by fluorescence in situ hybridization. Genomic DNA and mRNA were quantified using real-time quantitative PCR. Results: A novel amplification was found at the chromosomal region 1p32-31 in a JHH-2 HCC cell line within which JUN is amplified and overexpressed. However, no copy number gain of JUN (>2-fold) was observed in 34 primary HCC tumors. Rather, a loss of JUN (<0.5-fold) was seen in 13 (38%) out of the 34 tumors and expression of JUN was significantly lower in 26 (70%) out of the 37 HCC tumors compared with their nontumorous counterparts. Conclusion: Although JUN was amplified and overexpressed in JHH-2 HCC cells, amplification and overexpression of JUN may be rare in primary HCCs. %U https://ar.iiarjournals.org/content/anticanres/29/12/4989.full.pdf