PT - JOURNAL ARTICLE AU - ATSUSHI IDEO AU - KEN HASHIMOTO AU - JUN SHIMADA AU - MASAMI KAWASE AU - HIROSHI SAKAGAMI TI - Type of Cell Death Induced by α-Trifluoromethyl Acyloins in Oral Squamous Cell Carcinoma DP - 2009 Jan 01 TA - Anticancer Research PG - 175--181 VI - 29 IP - 1 4099 - http://ar.iiarjournals.org/content/29/1/175.short 4100 - http://ar.iiarjournals.org/content/29/1/175.full SO - Anticancer Res2009 Jan 01; 29 AB - We previously reported that α-trifluoromethyl acyloins (TFs) induced various types of cell death, depending on the target cancer cell line. We investigated here what type of cell death is induced by α-trifluoromethyl acyloins in two human oral squamous cell carcinoma cell lines (HSC-2, HSC-4). TFs produced few TUNEL-positive cells. TFs induced annexin V/PI-double positive HSC-2 cells and annexin V-positive/PI-negative HSC-4 cells, respectively, but failed to activate caspase-3, capase-8 and caspase-9 in both HSC-2 and HSC-4 cells. On the other hand, TFs induced the formation of acidic organelles (detected by acridine orange staining) in both HSC-2 and HSC-4 cells. When HSC-2 and HSC-4 cells that had been transfected with expression vector encording the microtubule-associated protein 1 light chain 3 (LC3) gene fused to green fluorescent protein (GFP) were treated with TFs, LC3-GFP fusion protein was accumulated as granular dots in autophagosomes. Pretreatment with 3-methyladenine, an inhibitor of autophagy, partially inhibited the cytotoxicity of TFs, the formation of acidic organelles and LC3 accumulation in the autophagosome. These data suggest that α-trifluoromethyl acyloins may induce autophagic cell death in HSC-2 and HSC-4 cells following the early stage of necrosis or apoptosis, respectively. Copyright© 2009 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved