RT Journal Article
SR Electronic
T1 An Immunocompetent Murine Model of Metastatic Mammary Cancer Accessible to Bioluminescence Imaging
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4389
OP 4395
VO 29
IS 11
A1 SHIBATA, MASA-AKI
A1 SHIBATA, EIKO
A1 MORIMOTO, JUNJI
A1 EID, NABIL A.S.
A1 TANAKA, YOSHIHISA
A1 WATANABE, MASAHITO
A1 OTSUKI, YOSHINORI
YR 2009
UL http://ar.iiarjournals.org/content/29/11/4389.abstract
AB Background: Many areas of research, including gene and pharmacological therapeutics, would benefit from longitudinal in vivo monitoring methodologies. To investigate the feasibility of one such methodology, we developed a murine mammary cancer model amenable to sequential bioluminescent imaging of tumor growth and metastasis in living animals. Materials and Methods: Metastatic mouse mammary carcinoma BJMC3879 cells were transfected to stably express firefly luciferase and inoculated into immunocompetent female BALB/c mice. Results: Sequential analysis using bioluminescent imaging showed increasing photon counts correlated to expanding mammary tumor volumes; in addition, strong signals from axillary, mandibular, femoral, thoracic and abdominal regions in mice were histopathologically determined to be due to metastases, the majority of which occurred in lymph nodes and lungs. Conclusion: The bioluminescent mouse mammary cancer model we established provides a method for quantifiable longitudinal in vivo imaging that can be used in gene and pharmacological therapy applications.