RT Journal Article
SR Electronic
T1 Establishment and Characterization of a Human Gastrointestinal Stromal Tumour (GIST) Xenograft in Athymic Nude Mice
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4331
OP 4336
VO 29
IS 11
A1 REVHEIM, MONA-ELISABETH
A1 SEIERSTAD, THERESE
A1 BERNER, JEANNE-MARIE
A1 BRULAND, ØYVIND SVERRE
A1 RØE, KATHRINE
A1 OHNSTAD, HEGE OMA
A1 BJERKEHAGEN, BODIL
A1 BACH-GANSMO, TORE
YR 2009
UL http://ar.iiarjournals.org/content/29/11/4331.abstract
AB Background: The majority of gastrointestinal stromal tumours (GISTs) contain oncogenic KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) or platelet-derived growth factor-alpha (PDGFRA) receptor tyrosine kinase (TK) mutations and are initially, but only temporarily sensitive to TK inhibitors. The aim of this study was to establish and characterize a human GIST xenograft that could be used for evaluating various molecularly targeted therapies. Materials and Methods: GIST tissue from four patients was implanted under the skin of athymic nude mice. In one case a tumour line was established. Results: The xenograft showed characteristic GIST morphology and exhibited the same mutation profile as that of the patient. Conclusion: A human GIST xenograft with mutation in KIT exons 11 and 17 has been established and maintained in nude mice for 3 years (13 passages). This model will enable further studies on mechanisms of resistance, combination therapies and allow testing of novel targeted therapies.