TY - JOUR T1 - Establishment and Characterization of a Human Gastrointestinal Stromal Tumour (GIST) Xenograft in Athymic Nude Mice JF - Anticancer Research JO - Anticancer Res SP - 4331 LP - 4336 VL - 29 IS - 11 AU - MONA-ELISABETH REVHEIM AU - THERESE SEIERSTAD AU - JEANNE-MARIE BERNER AU - ØYVIND SVERRE BRULAND AU - KATHRINE RØE AU - HEGE OMA OHNSTAD AU - BODIL BJERKEHAGEN AU - TORE BACH-GANSMO Y1 - 2009/11/01 UR - http://ar.iiarjournals.org/content/29/11/4331.abstract N2 - Background: The majority of gastrointestinal stromal tumours (GISTs) contain oncogenic KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) or platelet-derived growth factor-alpha (PDGFRA) receptor tyrosine kinase (TK) mutations and are initially, but only temporarily sensitive to TK inhibitors. The aim of this study was to establish and characterize a human GIST xenograft that could be used for evaluating various molecularly targeted therapies. Materials and Methods: GIST tissue from four patients was implanted under the skin of athymic nude mice. In one case a tumour line was established. Results: The xenograft showed characteristic GIST morphology and exhibited the same mutation profile as that of the patient. Conclusion: A human GIST xenograft with mutation in KIT exons 11 and 17 has been established and maintained in nude mice for 3 years (13 passages). This model will enable further studies on mechanisms of resistance, combination therapies and allow testing of novel targeted therapies. ER -