RT Journal Article
SR Electronic
T1 Preferential Expression of the Secreted and Membrane Forms of Tumor Endothelial Marker 7 Transcripts in Osteosarcoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4317
OP 4322
VO 29
IS 11
A1 CHANDRALEKHA HALDER
A1 CHRISTIAN OSSENDORF
A1 AVUDAIAPPAN MARAN
A1 MICHAEL YASZEMSKI
A1 MARK E. BOLANDER
A1 BRUNO FUCHS
A1 GOBINDA SARKAR
YR 2009
UL http://ar.iiarjournals.org/content/29/11/4317.abstract
AB Background: High expression of tumor endothelial marker 7 (TEM7) is correlated with osteogenic sarcoma (OS) metastasis and poor survival of patients. The TEM7 gene produces four alternatively spliced transcripts with distinct functional domains; the expression pattern of these transcripts in OS is unknown. Materials and Methods: mRNA expression was assessed in 5 OS cell lines, 7 normal bone, and 9 OS tumor specimens by reverse transcriptase polymerase chain reaction. Results: All OS cell lines, 6/9 tumors but none of the bone specimens expressed mRNA of TEM7 secreted forms 1 and 2. A total of 3/5 OS cell lines, 8/9 of tumors and 4/7 of bone specimens expressed mRNA of the TEM7 intracellular form. One out of 5 cell lines, 2/7 tumors and none of the bone specimens expressed mRNA of the TEM7 membrane form. The secreted forms had 20-fold higher expression in metastatic (LM7) compared to non-metastatic (SAOS-2) cells. Conclusion: The mRNA of secreted and the membrane forms of TEM7 are preferentially expressed in OS.