@article {HALDER4317, author = {CHANDRALEKHA HALDER and CHRISTIAN OSSENDORF and AVUDAIAPPAN MARAN and MICHAEL YASZEMSKI and MARK E. BOLANDER and BRUNO FUCHS and GOBINDA SARKAR}, title = {Preferential Expression of the Secreted and Membrane Forms of Tumor Endothelial Marker 7 Transcripts in Osteosarcoma}, volume = {29}, number = {11}, pages = {4317--4322}, year = {2009}, publisher = {International Institute of Anticancer Research}, abstract = {Background: High expression of tumor endothelial marker 7 (TEM7) is correlated with osteogenic sarcoma (OS) metastasis and poor survival of patients. The TEM7 gene produces four alternatively spliced transcripts with distinct functional domains; the expression pattern of these transcripts in OS is unknown. Materials and Methods: mRNA expression was assessed in 5 OS cell lines, 7 normal bone, and 9 OS tumor specimens by reverse transcriptase polymerase chain reaction. Results: All OS cell lines, 6/9 tumors but none of the bone specimens expressed mRNA of TEM7 secreted forms 1 and 2. A total of 3/5 OS cell lines, 8/9 of tumors and 4/7 of bone specimens expressed mRNA of the TEM7 intracellular form. One out of 5 cell lines, 2/7 tumors and none of the bone specimens expressed mRNA of the TEM7 membrane form. The secreted forms had 20-fold higher expression in metastatic (LM7) compared to non-metastatic (SAOS-2) cells. Conclusion: The mRNA of secreted and the membrane forms of TEM7 are preferentially expressed in OS.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/29/11/4317}, eprint = {https://ar.iiarjournals.org/content/29/11/4317.full.pdf}, journal = {Anticancer Research} }