PT - JOURNAL ARTICLE AU - CHIN-NAN CHU AU - KING-CHUEN WU AU - WAI-SHAN CHUNG AU - LI-CHENG ZHENG AU - TA-KUO JUAN AU - YUNG-TING HSIAO AU - SHU-FEN PENG AU - JUNG-LONG YANG AU - YI-SHIH MA AU - RICK SAI-CHUEN WU AU - JING-GUNG CHUNG TI - Etomidate Suppresses Invasion and Migration of Human A549 Lung Adenocarcinoma Cells AID - 10.21873/anticanres.13100 DP - 2019 Jan 01 TA - Anticancer Research PG - 215--223 VI - 39 IP - 1 4099 - http://ar.iiarjournals.org/content/39/1/215.short 4100 - http://ar.iiarjournals.org/content/39/1/215.full SO - Anticancer Res2019 Jan 01; 39 AB - Background/Aim: Etomidate, an intravenous anesthetic, has been shown to have anticancer effects, including induction of cell-cycle arrest and apoptosis. However, to our knowledge, there are no reports about the anti-metastasis effects of etomidate on A549 human lung adenocarcinoma cells. Materials and Methods: The cell viability, cell adhesion, gelatin zymography assay, transwell migration and invasion assay, and western blotting analysis were used to investigate the effects of etomidate on A549 cells. Results: In our study, etomidate showed low cytotoxicity, inhibited cell adhesion, and suppressed the migration and invasion in A549 cells. The activity of matrix metallopeptidase 2 (MMP2) was reduced by 48 h treatment of etomidate. Results of western blotting analysis indicated that etomidate down-regulated the expression of protein kinase C, MMP7, MMP1, MMP9, and p-p-38, but up-regulated that of RAS, phosphoinositide 3-kinase, and phosphor-extracellular-signal related kinase after 24 and 48 h treatment, in A549 cells. Conclusion: Etomidate suppressed the migration and invasion of lung adenocarcinoma A549 cells via inhibiting the expression of MMP1, MMP2, MMP7 and MMP9, and provides potential therapeutic targets for lung cancer treatment.