RT Journal Article
SR Electronic
T1 Correspondence between Flat Aberrant Crypt Foci and Mucin-depleted Foci in Rodent Colon Carcinogenesis
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3771
OP 3775
VO 28
IS 6A
A1 FEMIA, ANGELO PIETRO
A1 PAULSEN, JAN ERIK
A1 DOLARA, PIERO
A1 ALEXANDER, JAN
A1 CADERNI, GIOVANNA
YR 2008
UL http://ar.iiarjournals.org/content/28/6A/3771.abstract
AB Background: Flat aberrant crypt foci (flat ACF) and mucin-depleted foci (MDF) are preneoplastic lesions identified in the colon of carcinogen-treated rodents stained with methylene blue (MB) and high iron diamine-alcian blue (HID-AB), respectively. The correspondence between flat ACF and MDF in the same colon of Min mice treated with azoxymethane (AOM) and of F344 rats treated with 1,2-dimethylhydrazine (DMH) was explored. Materials and Methods: The position of each flat ACF was recorded on a digitally constructed photographic map of the MB-stained colon. The same colons were then stained with HID-AB and the position of each MDF was compared with that of flat ACF. Results: The fraction of coincident lesions, identified as both flat ACF and MDF with the two staining methods, was 57% and 42%, in the Min mice and F344 rats, respectively. Flat ACF or MDF not coincident with the two staining methods were either undetectable or ACF with one of the two methods. Conclusion: Flat ACF and MDF show considerable, but not total, overlap. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved