RT Journal Article SR Electronic T1 Tumor-specific Cytotoxicity and Type of Cell Death Induced by Peplomycin in Oral Squamous Cell Carcinoma Cell Lines JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 2197 OP 2204 VO 28 IS 4B A1 YU LIU A1 HIROSHI SAKAGAMI A1 OSAMU AMANO A1 HIROTAKA KIKUCHI A1 YUKIO NAKAMURA A1 MARIKO ISHIHARA A1 YUMIKO KANDA A1 SHIRO KUNII A1 WEI ZHANG A1 GUANGYAN YU YR 2008 UL http://ar.iiarjournals.org/content/28/4B/2197.abstract AB The antitumor antibiotic peplomycin showed higher cytostatic antiproliferative effect on five cultured human oral squamous cell carcinoma (OSCC) cell lines (HSC-2, HSC-3, HSC-4, Ca9-22 and NA), as compared with three human oral normal cells (gingival fibroblast HGF, pulp cell HPC and periodontal ligament fibroblast HPLF). Although the antiproliferative activity of peplomycin declined with increasing cell density, peplomycin showed tumor-specific cytotoxicity at any cell density. The five OSCC cell lines showed considerable differences in sensitivity against peplomycin; the HSC-2 cells were the most sensitive, followed by the NA, HSC-3, Ca9-22 and HSC-4 cells. Peplomycin did not induce internucleosomal DNA fragmentation in any of the five OSCC cell lines, and only slightly modified caspase-3, -8 and -9 activities in the HSC-2, Ca9-22 and NA cell lines. Electron microscopy revealed that peplomycin induced the vacuolation of mitochondria accompanying electron lucent matrices lacking cristae and the enlargement of the endoplasmic reticulum in the HSC-2 cells. These data suggest that the anti-proliferative effect of peplomycin is time-dependent, and therefore prolonged treatment with peplomycin in combination with cytotoxic chemotherapeutic agents may induce greater cytotoxic action. Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved